Abstract
This study focused on the legacy effects of 8-year application of N (in gradient of 0, 140, 280, 470, and 660 kg N ha−1 year−1) on the bacterial community diversity, interactions, and assembly processes in the wheat rhizosphere. The rhizosphere bacterial α-diversity increased with the rate of historical N input, while it did not change at N addition rates of over 280 kg N ha−1 year−1. Historical N input clearly shifted the rhizosphere bacterial community composition, and soils with more N input were more dissimilar to those without N input. The net relatedness index (NRI) and nearest taxon index (NTI) analysis revealed that the rhizosphere bacterial communities in most samples were phylogenetically clustered, and the treatments with high N (> 470 kg N ha−1 year−1) showed higher levels of clustering than those with low N (< 140 kg N ha−1 year−1), indicating more environmental selection stress in soil with higher historical N input. Increased co-occurrence network size and connectivity were accompanied by increased aboveground biomass of wheat. Overall, with the increase in historical N input, the resulting legacy effects forced the bacterial community in the rhizosphere to undergo higher environmental selection pressure, and indirectly affected the complexity of wheat rhizosphere assemblages during subsequent crop growth.
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Specially, we are grateful to Lauri Mikonranta for the helpful comments that helped us to improve the manuscript.
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This research was financially supported by the National Key Research and Development Program of China (2017YFD0200206), the Special Fund for Agro-scientific Research in the Public Interest (20150312205), the Natural Science Foundation of Jiangsu Province (BK20190543), the China Postdoctoral Science Foundation (2019M651861), the Innovative Research Team Development Plan of the Ministry of Education of China (IRT_17R56), and the Fundamental Research Funds for the Central Universities (KYT201802).
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Liu, W., Ling, N., Guo, J. et al. Legacy effects of 8-year nitrogen inputs on bacterial assemblage in wheat rhizosphere. Biol Fertil Soils 56, 583–596 (2020). https://doi.org/10.1007/s00374-020-01435-2
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DOI: https://doi.org/10.1007/s00374-020-01435-2