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Long-term and legacy effects of manure application on soil microbial community composition

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Abstract

We analyzed soil prokaryotic and fungal community composition in soils with varying histories of cattle manure application. The manure treatments were (i) annual application for 43 years (MF), (ii) annual application for 14 years followed by 29 years without application (MF14), and (iii) annual application for 30 years followed by 13 years without application (MF30). An annual application of chemical nitrogen (N) fertilizer (CNF) and a non-amended control (Con) were also included. Soil prokaryotic evenness and diversity significantly decreased in MF relative to other treatments in fall, but were similar to the other fertilizer treatments in spring and summer. Distinct prokaryotic and fungal community composition was observed in MF compared to other treatments across fall, spring, and summer seasons. The MF treatment significantly increased the relative abundance of Firmicutes, Gammaproteobacteria, and Gemmatimonadetes, but significantly decreased the relative abundance of Acidobacteria. In fall, the soil prokaryotic and fungal community composition with MF30 was significantly different than the other fertilization treatments. Overall, the study showed that annual manure application (MF) led to a different microbial community composition than the other fertilizer treatments. Soil without manure application for 13 years (MF30) had a significantly different microbial community composition from other fertilizer treatments in fall, while the soil without manure application for 29 years (MF14) resembled a microbial community that had never received manure.

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Acknowledgements

We are grateful to Drs T. Sommerfeldt and C. Chang for initiating this long-term manure trial, and to the technical staff and field crew for field operations. We thank Agriculture & Agri-Food Canada for funding this study under the Growing Forward 2 program.

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Authors and Affiliations

  1. College of Resources and Environment, Southwest University, Chongqing, 400716, China

    Yuting Zhang & Xiaojun Shi

  2. Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, T1J4B1, Canada

    Yuting Zhang, Xiying Hao, Trevor W. Alexander, Ben W. Thomas & Newton Z. Lupwayi

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  1. Yuting Zhang
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Correspondence to Newton Z. Lupwayi.

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Zhang, Y., Hao, X., Alexander, T.W. et al. Long-term and legacy effects of manure application on soil microbial community composition. Biol Fertil Soils 54, 269–283 (2018). https://doi.org/10.1007/s00374-017-1257-2

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