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Impact of 12-year field treatments with organic and inorganic fertilizers on crop productivity and mycorrhizal community structure

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Abstract

The effect of manure and mineral fertilization on the arbuscular mycorrhizal (AM) fungal community structure of sunflower (Helianthus annuus L.) plants was studied. Soils were collected from a field experiment treated for 12 years with equivalent nitrogen (N) doses of inorganic N, dairy manure slurry, or without N fertilization. Fresh roots of tall fescue (Festuca arundinacea Schreb.) grass collected from the field plots without N fertilization and unfumigated field soils were used as native microbial inoculum sources. Sunflower plants were sown in pots containing these soils, and three different means of manipulating the microbial community were set: unfumigated soil with fresh grass roots, fumigated soil with fresh grass roots, or fumigated soil with sterilized grass roots. Assessing the implications with respect to plant productivity and mycorrhizal community structure was investigated. Twelve AM fungal OTUs were identified from root or soil samples as different taxa of Acaulospora, Claroideoglomus, Funneliformis, Rhizophagus, and uncultured Glomus, using PCR-DGGE and sequencing of an 18S rRNA gene fragment. Sunflower plants grown in manure-fertilized soils had a distinct AMF community structure from plants either fertilized with mineral N or unfertilized, with an abundance of Rhizophagus intraradices-like (B2). The results also showed that AM inoculation increased P and N contents in inorganic N-fertilized or unfertilized plants, but not in manure-fertilized plants.

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Acknowledgments

This work was supported by NSERC discovery grants to both MSA and MH and by a fellowship from the Ministry of Higher Education of Egypt to SEH, to which supports are gratefully acknowledged. Funding was also provided by NSERC Metal in the Human Environment (MITHE) grant. We thank Stéphane Daigle for helping with statistical analysis and Cristina Micali for comments and English editing.

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Authors and Affiliations

  1. Institut de recherche en biologie végétale, Université de Montréal and Jardin botanique de Montréal, 4101 Sherbrooke St. East, Montreal, QC, H1X 2B2, Canada

    Saad El Din Hassan, Mohamed Hijri & Marc St-Arnaud

  2. Botany & Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt

    Saad El Din Hassan

  3. Agriculture and Agri-Food Canada, P.O. Box 1000, 6947 Highway 7, Agassiz, BC, V0M 1A0, Canada

    Aiguo Liu, Shabtai Bittman, Thomas A. Forge & Derek E. Hunt

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  1. Saad El Din Hassan
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  2. Aiguo Liu
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  4. Thomas A. Forge
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  6. Mohamed Hijri
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  7. Marc St-Arnaud
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Correspondence to Mohamed Hijri or Marc St-Arnaud.

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Hassan, S.E.D., Liu, A., Bittman, S. et al. Impact of 12-year field treatments with organic and inorganic fertilizers on crop productivity and mycorrhizal community structure. Biol Fertil Soils 49, 1109–1121 (2013). https://doi.org/10.1007/s00374-013-0813-7

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  • DOI: https://doi.org/10.1007/s00374-013-0813-7

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