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Evidence for a membrane-bound carbonic anhydrase in the heart of an ancient vertebrate, the sea lamprey (Petromyzon marinus)

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Abstract

In order to gain insight into the early evolution of carbonic-anhydrase (CA) isozymes in vertebrates, the main objective of the present study was to determine whether the hearts of an ancient vertebrate species, Petromyzon marinus, possess a membrane-bound CA isozyme. Since a significant amount of CA activity appeared to be strongly associated with the heart membrane fraction after differential centrifugation and washing, further experiments were conducted to examine the inhibitor properties of the CA from the membrane fraction in comparison with lamprey cytoplasmic CA from the red blood cell (rbc) fraction. These experiments showed that the inhibitor properties of the CA from the heart membranes were significantly different from those of the cytoplasmic CA from lamprey rbcs. A final series of experiments showed that the membrane-bound CA in the lamprey heart is not anchored via a glycosylphosphatidylinositol (GPI) linkage. Taken together, the results of these studies indicate that a membrane-bound CA does appear to be present in the hearts of lamprey, but the properties of the membrane-bound CA isozyme in these ancient vertebrates appear to differ from those in more recently evolved groups.

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Abbreviations

Az :

acetazolamide

CA :

carbonic anhydrase

GPI :

glycosylphosphatidylinositol

PI-PLC :

phosphatidylinositol specific phospholipase C

Rbc :

red blood cell

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Acknowledgements

Financial support for this study was provided by an NSERC grant to BLT. AJE was supported by a OGS and NSERC graduate scholarships. The authors also gratefully acknowledge the Lamprey Control Center of the Department of Fisheries and Oceans in Sault Ste. Marie, Ontario, for their logistical support in obtaining experimental animals for this study.

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  1. Department of Biology, Queen’s University, Kingston, Ontario, K7L 3N6, Canada

    A. J. Esbaugh & B. L. Tufts

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  1. A. J. Esbaugh
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  2. B. L. Tufts
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Correspondence to A. J. Esbaugh.

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Esbaugh, A.J., Tufts, B.L. Evidence for a membrane-bound carbonic anhydrase in the heart of an ancient vertebrate, the sea lamprey (Petromyzon marinus). J Comp Physiol B 174, 399–406 (2004). https://doi.org/10.1007/s00360-004-0426-4

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  • DOI: https://doi.org/10.1007/s00360-004-0426-4

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