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Elevated Expression of YSL Genes and Enhanced Physiological Responses in Rice Subjected to Iron Application

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Abstract

The study delves into the vital role of essential metal nutrient uptake in graminaceous plants, focusing on internal movement for optimal plant development. The experiment specifically explores the impact of iron application in soil with a pH of 7.5. Findings reveal a notable increase in iron (Fe) content in Oryza sativa post iron application. Specifically, Bas-198 displayed a noteworthy 109.09% increase, while Bas-515 exhibited a remarkable 119.05% increase in total iron accumulation per plant at the highest concentration. In contrast to Bas-515, Bas-198 demonstrated notable enhancements across various parameters, including a 91% increase in root dry weight, a 71.43% increase in net photosynthesis, a 115% increase in CAT activity, a 75% increase in APX activity, a substantial 180% increase in POD activity, and an impressive 150% increase in SOD. At 500 mM, Basmati-198 experienced significant decreases of 55.71%, 64.29%, and 70.73% in MDA, H2O2, and EL content, respectively. Conversely, Basmati-515 demonstrated a substantial decrease of 53.85%, 50%, and 50% in MDA, H2O2, and EL, respectively. YSL genes, particularly OsYSL8 and OsYSL9, highly responsive to iron deficiency in rice, were extensively characterized. In Bas-515, gene expression surged significantly, reaching 5.5 times its initial level, while in Bas-198, it exhibited a remarkable increase to 6.7 times. For YSL-9, substantial upregulation was evident at 500 mM, with gene expression increasing to 4.2 times and 5.1 times in Bas-515 and Bas-198, respectively. Insights derived from this study provide valuable guidance for the identification of genes involved in iron uptake and the refinement of both iron application and RNA extraction strategies. These findings contribute to ongoing efforts aimed at enhancing plant nutrient uptake efficiency, ultimately advancing our understanding of key mechanisms governing iron assimilation in plants.

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Authors and Affiliations

  1. Institute of Botany, University of the Punjab, Lahore, Pakistan

    Ammara Saleem & Asma Zulfiqar

  2. Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan

    Muhammad Zafar Saleem

  3. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Muhammad Hamzah Saleem

  4. Department of Agronomy, Abdul Wali Khan University Mardan, Mardan, 23200, Khyber Pakhtunkhwa, Pakistan

    Shah Fahad

  5. Department of Natural Sciences, Lebanese American University, Byblos, Lebanon

    Shah Fahad

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  1. Ammara Saleem
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  2. Asma Zulfiqar
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  4. Muhammad Hamzah Saleem
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AS: Investigation, Methodology, Data curation, Supervision, Writing—original draft, Funding acquisition. AZ: Investigation, Methodology, Writing—original draft. MZS: Investigation, Methodology. MHS: Formal analysis, Investigation. SF: Data curation, Writing—review and editing.

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Saleem, A., Zulfiqar, A., Saleem, M.Z. et al. Elevated Expression of YSL Genes and Enhanced Physiological Responses in Rice Subjected to Iron Application. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11249-3

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