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Gene expression and activity of antioxidant enzymes in rice plants, cv. BRS AG, under saline stress

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Abstract

The rice cultivar (Oryza sativa L.) BRS AG, developed by Embrapa Clima Temperado, is the first cultivar designed for purposes other than human consumption. It may be used in ethanol production and animal feed. Different abiotic stresses negatively affect plant growth. Soil salinity is responsible for a serious reduction in productivity. Therefore, the objective of this study was to evaluate the gene expression and the activity of antioxidant enzymes (SOD, CAT, APX and GR) and identify their functions in controlling ROS levels in rice plants, cultivar BRS AG, after a saline stress period. The plants were grown in vitro with two NaCl concentrations (0 and 136 mM), collected at 10, 15 and 20 days of cultivation. The results indicated that the activity of the enzymes evaluated promotes protection against oxidative stress. Although, there was an increase of reactive oxygen species, there was no increase in MDA levels. Regarding genes encoding isoforms of antioxidant enzymes, it was observed that OsSOD3-CU/Zn, OsSOD2-Cu/Zn, OsSOD-Cu/Zn, OsSOD4-Cu/Zn, OsSODCc1-Cu/Zn, OsSOD-Fe, OsAPX1, OsCATB and OsGR2 were the most responsive. The increase in the transcription of all genes among evaluated isoforms, except for OsAPX6, which remained stable, contributed to the increase or the maintenance of enzyme activity. Thus, it is possible to infer that the cv. BRS AG has defense mechanisms against salt stress.

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Acknowledgements

This work was supported by the Brazilian funding agency Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Instituto de Biologia, Departamento de Botânica, Universidade Federal de Pelotas, Campus Universitário, Capão do Leão, RS, 96160-000, Brazil

    Tatiana Rossatto, Marcelo Nogueira do Amaral, Letícia Carvalho Benitez, Isabel Lopes Vighi & Eugenia Jacira Bolacel Braga

  2. Embrapa Clima Temperado, Pelotas, RS, 96001-970, Brazil

    Ariano Martins de Magalhães Júnior

  3. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Laboratório de Bioinformática e Proteômica (BioPro_Lab), Universidade Federal de Pelotas, Campus Universitário, Capão do Leão, RS, 96160-000, Brazil

    Mara Andrade Colares Maia & Luciano da Silva Pinto

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  1. Tatiana Rossatto
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  2. Marcelo Nogueira do Amaral
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  3. Letícia Carvalho Benitez
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  6. Ariano Martins de Magalhães Júnior
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  8. Luciano da Silva Pinto
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Contributions

Conceived and designed the experiments LSP, LCB and AMMJ. Wrote, edited and analyzed the data: TR, LCB, MNA and ILV. Revised the paper: LSP, AMMJ and EJBB. Conducted the experiments: TR, LCB, MNA and ILV. All authors readed the paper and approved the final manuscript.

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Correspondence to Tatiana Rossatto.

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Rossatto, T., do Amaral, M.N., Benitez, L.C. et al. Gene expression and activity of antioxidant enzymes in rice plants, cv. BRS AG, under saline stress. Physiol Mol Biol Plants 23, 865–875 (2017). https://doi.org/10.1007/s12298-017-0467-2

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  • DOI: https://doi.org/10.1007/s12298-017-0467-2

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