Abstract
Heavy metals affect human health through the physical contact with polluted soil, polluted water, and food contaminated with toxic metals. Cadmium (Cd) toxicological effects remain for many years in the ecosystem. In this study, in vitro growth of banana (Musa acuminata) shoots was evaluated under different concentrations of Cd (0, 50, 100, 200, 500 and 1000 μM) on MS gelled medium for two subsequent subcultures. Also, salicylic acid (SA) was studied as a pretreatment to assess Cd tolerance in banana shoots, using different concentrations (0, 0.5 and 1.0 mM). Cd toxicity decreased plantlet height, as well as fresh and dry biomass at all concentrations evaluated. The highest reduction was observed at the 2nd subculture (2S) on Cd medium at 200 μM, ending with shoot death at 500 μM. However, SA pretreated banana shoots exhibited a better performance, even in 2S and under the culture in high Cd-containing media, compared to non-pretreated shoots, and this protective effect was higher at the SA concentration of 0.5 μM than at 1 μM. The high performance due to 0.5 mM SA included shoots survival, plantlet length, biomass, photosynthetic pigments, and proline content. To our knowledge, this is the first study to examine the effect of Cd on in vitro banana shoots for two successive subcultures, as well as to give a significant indication of the role of SA as a pretreatment in alleviating the toxicity of Cd, even at high concentrations. Findings of this study concluded that SA has a great and extended influence on reducing the harmful effects of Cd in banana.
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DSE designed and carried out the experimental design, data collection, manuscript writing and helped in preparing the manuscript. MTE helped with designing the experiment, data collection and also helped in writing the manuscript. MY contributed with data analysis and tables and figures preparing. MAA carried out the metal analysis. AA helped in writing the manuscript. ML helped in data analysis and carried out the final writing and total revision for the manuscript. All authors read and revised the manuscript.
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Elazab, D.S., El-Mahdy, M., Youssef, M. et al. Assessment of Salicylic Acid as a Pretreatment on Alleviating Cadmium Toxicity on In Vitro Banana Shoots. J Plant Growth Regul 42, 5700–5712 (2023). https://doi.org/10.1007/s00344-023-10951-y
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DOI: https://doi.org/10.1007/s00344-023-10951-y