Abstract
Achnatherum inebrians is rich in alkaloids (ergonovine and ergine) with analgesic activities and insect resistance activities and prevents animal feeding. Many studies have focused on the effects of abiotic stresses on alkaloid accumulation. However, the ergot alkaloids differences of plants from environmentally distinct sites remain unclear. The Epichloë endophyte usually regulates plant hormone pathways; meanwhile, JA and SA indirectly manipulate the production of metabolites, while the relationship between the concentrations of ergot alkaloids and endogenous hormones still lacks advanced studies. The present study identified ergot alkaloids and endogenous hormones of A. inebrians from different sites using HPLC and ELISA, respectively. The original geographical location of the germplasm determines the change of alkaloid and hormone content. Redundancy analysis indicated that the altitude and mean month temperature were the major factors affecting alkaloids and endogenous hormones levels. Principal component analysis indicated that there were difference in the relationship between alkaloids and endogenous hormones at low and high altitude. These results improve our understanding of the variation in ergot alkaloids of A. inebrians from different sites and will serve as a reference for evaluating the medicinal, forage, slope protection grass, and airport turfgrass value of A. inebrians in different environments. In addition, it provides a new understanding of the relationship among altitude with ergot alkaloids and endogenous hormones.
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This research was supported by the National Basic Research Program of China (2014CB138702), the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (2019QZKK0302), Program for Changjiang Scholars and Innovative Research Team in University, China (IRT17R50), Fundamental Research Funds for the Central Universities (LZUJBKY- 2020-it11), and 111 Project (B12002). The authors are thankful for support from USDA-NIFA Multistate Project W4147 and the New Jersey Agricultural Experiment Station.
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JL, XZL, YYJ and ZJC outlined the experimental setup and analyzed the data. JL wrote the initial draft of the manuscript. ZJC, TXC, QS, JFW and CJL revised the manuscript to the present form. The manuscript was read by all authors and approved for submission.
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Liu, J., Chen, Z., White, J.F. et al. Ergot Alkaloid and Endogenous Hormones Quantities and Relationship in Epichloë Endophyte: Drunken Horse Grass are Affected by Altitude. J Plant Growth Regul 42, 1979–1990 (2023). https://doi.org/10.1007/s00344-022-10675-5
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DOI: https://doi.org/10.1007/s00344-022-10675-5