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In Vitro Propagation Using Transverse Thin Cell Layer Culture and Homogeneity Assessment in Ceropegia bulbosa Roxb.

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Abstract

Ceropegia bulbosa is an endangered medicinal plant used traditionally in the treatment of various diseases. Our aim is to develop a rapid and a competent procedure for direct and indirect organogenesis from transverse thin cell layer (tTCL) explants of C. bulbosa. Optimum response to direct adventitious shoot bud induction from tTCLs was observed on medium augmented with 8.8 µM 6-benzyladenine (BA) producing 15.6 ± 0.31 shoots per responsive explant. Best callusing response (95 %) was observed with tTCL explants in medium containing 4.5 µM 2,4-dichlorophenoxyacetic acid and 2.2 µM BA. High frequency shoot regeneration (75 %) was observed from tTCL derived calli. Medium containing 8.8 µM BA and 0.27 µM α-naphthalene acetic acid produced 22.2 ± 0.64 shoots with shoots acquiring an average length of 4.6 ± 0.12 cm. In vitro rooting was recorded on ½ strength Murashige and Skoog medium, producing 10.9 ± 0.23 roots with a length of 4.24 ± 0.16 cm. Plants were successfully transferred to the field with a survival rate of 89 %. The clonal nature of the regenerants was assessed using Inter-simple sequence repeat markers.

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Acknowledgments

G. S. Shekhawat acknowledges financial support from Department of Science and Technology (Raj.) and also thankful to University Grants Commission (New Delhi) for the support provided through Center for Advance Study.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India

    Richa Dhir

  2. Department of Botany, Jai Narain Vyas University, Jodhpur, 342001, Rajasthan, India

    Gyan Singh Shekhawat

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  1. Richa Dhir
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  2. Gyan Singh Shekhawat
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Correspondence to Gyan Singh Shekhawat.

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Dhir, R., Shekhawat, G.S. In Vitro Propagation Using Transverse Thin Cell Layer Culture and Homogeneity Assessment in Ceropegia bulbosa Roxb.. J Plant Growth Regul 33, 820–830 (2014). https://doi.org/10.1007/s00344-014-9432-2

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  • DOI: https://doi.org/10.1007/s00344-014-9432-2

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