Abstract
The effects of cytokinins on somatic embryogenesis in pepper were studied using a Murashige and Skoog-based medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 10% sucrose. Compared to the medium without cytokinins, N6-(Δ2-isopentenyl)-adenine and kinetin had no significant effect, whereas benzylaminopurine and zeatin significantly reduced somatic embryogenesis. Coconut water (10%) either had no significant effect or it reduced embryogenesis as well. Induction of somatic embryogenesis was also dependent on genotype. Appearance of somatic embryos continued after removal of the embryos that developed first, especially on cultivars with a lower initial induction rate. Although somatic embryos germinated, the apical meristem did not elongate.
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References
Binzel ML, Sankhla N, Joshi S, Sankhla D (1996) Induction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.). Plant Cell Rep 15:536–540
Bodhipadma K, Leung DWM (2002) Factors important for somatic embryogenesis in zygotic embryo explants of Capsicum annuum L. J Plant Biol 45:49–55
Bodhipadma K, Leung DWM (2003) Histological changes during direct somatic embryogenesis from immature zygotic embryo explants of Capsicum annuum L. cv. Sweet Banana. Phyton-Ann Rei Bot 43:197–206
Coenen C, Lomax TL (1997) Auxin-cytokinin interactions in higher plants: old problems and new tools. Trends Plant Sci 2:351–356
Ge LY, Yong JWH, Tan SN, Ong ES (2006) Determination of cytokinins in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry. Electrophoresis 27:2171–2181
Harini I, Sita GL (1993) Direct somatic embryogenesis and plant regeneration from immature embryos of chilli (Capsicum annuum L.). Plant Sci 89:107–112
Holden PR, Yeoman MM (1994) Variation in the growth and biosynthetic activity of cloned cell cultures of Capsicum frutescens and their response to an exogenously supplied elicitor. Plant Cell Tiss Org Cult 38:31–37
Jo JY, Choi EY, Choi D, Lee KW (1996) Somatic embryogenesis and plant regeneration from immature zygotic embryo culture in pepper (Capsicum annuum L.). J Plant Biol 39:127–135
Komamine A, Kawahara R, Matsumoto M, Sunabori S, Toya T, and others (1992) Mechanisms of somatic embryogenesis in cell cultures: Physiology, biochemistry and molecular biology. In Vitro Cell Dev Biol-Plant 28:11–14
Mok MC, Mok DWS, Armstrong DJ (1978) Differential cytokinin structure-activity relationships in Phaseolus. Plant Physiol 61:72–75
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ochoa-Alejo N, Ramirez-Malagon R (2001) Invited review: In vitro chili pepper biotechnology. In Vitro Cell Dev Biol Plant 37:701–729
Orr JD, Lynn DG (1992) Biosynthesis of dehydrodiconiferyl alcohol glucosides: implications for the control of tobacco cell growth. Plant Physiol 98:343–352
Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol 11:118–131
Steinitz B, Kusek M, Tabib Y, Paran I, Zelcer A (2003) Pepper (Capsicum annuum L.) regenerants obtained by direct somatic embryogenesis fail to develop a shoot. In Vitro Cell Dev Biol Plant 39:296–303
Whitty CD, Hall RH (1974) A cytokinin oxidase in Zea mays. Can J Biochem 52:789–799
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Kaparakis, G., Alderson, P.G. Role for Cytokinins in Somatic Embryogenesis of Pepper (Capsicum annuum L.)?. J Plant Growth Regul 27, 110–114 (2008). https://doi.org/10.1007/s00344-007-9037-0
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DOI: https://doi.org/10.1007/s00344-007-9037-0