Abstract
Myostatin (MSTN) is a member of the transforming growth factor-β gene superfamily that negatively regulates skeletal muscle development and growth. In the present study, partial genomic fragments of Myostatin-1 (MSTN-1) in two commercial hatchery populations of Ancherythroculter nigrocauda, an economically important freshwater fish, were screened for single nucleotide polymorphisms (SNPs) and then genotyped by direct sequencing of PCR products. Five SNPs were identified in intron 1 and exon 2, including a non-synonymous mutation causing an amino acid change (Val to Ile) at position 180. Association analyses based on 300 individuals revealed that the g.1129T>C SNP locus was significantly associated with total length (TL), body length (BL), body height (BH) and body weight (BW) in 6- and 18-month-old populations, while the g.1289G>A locus was significantly associated with BH and BW in the 6-month-old population. Haplotype analyses revealed that fish with the genotype combinations TC/TC or TC/GA showed better growth performance. Our results suggest that g.1129T>C and g.1289G>A have positive effects on growth traits and may be candidate gene markers for marker-assisted selection in A. nigrocauda.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boman I A, Klemetsdal G, Blichfeldt T, Nafstad O, Våge D I. 2009. A frameshift mutation in the coding region of the myostatin gene (MSTN) affects carcass conformation and fatness in Norwegian white sheep (Ovis aries). Anim. Genet., 40 (4): 418–422.
De-Santis C, Jerry D R. 2007. Candidate growth genes in finfish-where should we be looking? Aquaculture, 272 (1-4): 22–38.
Fontanesi L, Scotti E, Frabetti A, Fornasini D, Picconi A, Russo V. 2011. Identification of polymorphisms in the rabbit (Oryctolagus cuniculus) myostatin (MSTN) gene and association analysis with finishing weight in a commercial rabbit population. Anim. Genet., 42 (3): 339.
Garikipati D K, Gahr S A, Rodgers B D. 2006. Identification, characterization, and quantitative expression analysis of rainbow trout myostatin-1a and myostatin-1b genes. J. Endocrinol., 190 (3): 879–888.
Guo L, Li L, Zhang S, Guo X, Zhang G. 2011. Novel polymorphisms in the myostatin gene and their association with growth traits in a variety of bay scallop, Argopecten irradians. Anim. Genet., 42 (3): 339–340.
Gutierrez A P, Lubieniecki K P, Davidson E A, Lien S, Kent M P, Fukui S, Withler R E, Swift B, Davidson W S. 2012. Genetic mapping of quantitative trait loci (QTL) for bodyweight in Atlantic salmon (Salmo salar) using a 6.5 K SNP array. Aquaculture, 358-359: 61–61.
Hayes B, Baranski M, Goddard M E, Robinson N. 2007. Optimisation of marker assisted selection for abalone breeding programs. Aquaculture, 265 (1-4): 61–69.
He F, Wen H S, Dong S L, Shi B, Chen C F, Wang L S, Yao J, Mu X J, Zhou Y G. 2008. Identification of single nucleotide polymorphism cytochrome P450-c19a and its relation to reproductive traits in Japanese flounder (Paralichthys olivaceus). Aquaculture, 279 (1-4): 177–181.
He X P, Xia J H, Wang C M, Pang H Y, Yue G H. 2010. Significant associations of polymorphisms in the prolactin gene with growth traits in Asian seabass (Lates calcarifer). Anim. Genet., 43 (2): 233–236.
Liu L S, Yu X M, Tong J G. 2012. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis). Mol. Biol. Rep., 39 (9): 9211–9221.
Liu Z J, Cordes J F. 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238 (1-3): 1–37.
Luo Y Y. 1998. Cultrinae. In: Chen Y Y ed. Fauna Sinica: Osteichthyes: Cypriniformes II. Sciences Press, Beijing, China. p. 112–207. (in Chinese)
McPherron A C, Lawler A M, Lee S J. 1997. Regulation of skeletal muscle mass in mice by a new TGF-p superfamily member. Nature, 387 (6628): 83–90.
McPherron A C, Lee S J. 1997. Double muscling in cattle due to mutations in the myostatin gene. P roc. Natl. Acad. Sci. USA, 94 (23): 12457–12461.
Østbye T K K, Wetten O F, Tooming-Klunderud A, Jakobsen K S, Yafe A, Etzioni S, Moen T, Andersen Ø. 2007. Myostatin (MSTN) gene duplications in Atlantic salmon (Salmo salar): evidence for different selective pressure on teleost MSTN -1 and -2. Gene, 403 (1-2): 159–169.
Peñaloza C, Hamilton A, Guy D R, Bishop S C, Houston R D. 2013. A SNP in the 5’ flanking region of the myostatin-1b gene is associated with harvest traits in Atlantic salmon (Salmo salar). BMC Genet., 14: 112.
Spelman R J, Garrick D J, Van Arendonk J A M. 1999. Utilisation of genetic variation by marker assisted selection in commercial dairy cattle populations. Livestock Production Science, 59 (1): 51–60.
Sun Y H, Yu X M, Tong J G. 2012. Polymorphisms in Myostatin gene and associations with growth traits in the common carp (Cyprinus carpio L.). International Journal of Molecular Sciences, 13 (12): 14956–14961.
Tang Y K, Li J L, Yu J H, Chen X F, Li H X. 2010. Genetic structure of MSTN and association between its polymorphisms and growth traits in genetically improved farmed tilapia (GIFT). Journal of Fishery Sciences of China, 17 (1): 44–51. (in Chinese with English abstract)
Tao W J, Boulding E G. 2010. Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr (Salvelinus alpinus L.). Heredity, 91 (1): 60–69.
Tian C X, Yang M, Lv L Y, Yuan Y C, Liang X F, Guo W J, Song Y, Zhao C. 2014. Single nucleotide polymorphisms in Growth Hormone gene and their association with growth traits in Siniperca chuatsi (Basilewsky). Int. J. Mol. Sci., 15 (4): 7029–7036.
Tong J G, Sun X W. 2015. Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish. Sci ence China Life Sci ences, 58 (2): 178–186.
Vignal A, Milan D, Sancristobal M, Eggen A. 2002. A review on SNP and other types of molecular markers and their use in animal genetics. Genet. Sel. Evol., 34 (3): 275–305.
Wang X L, Meng X Y, Song B, Qiu X M, Liu H Y. 2010. SNPs in the myostatin gene of the mollusk Chlamys farreri: association with growth traits. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 155 (3): 327–330.
Wang Y, Wang X L, Meng X Y, Wang H D, Jiang Z Q, Qiu X M. 2014. Identification of two SNPs in myostatin (MSTN) gene of Takifugu rubripes and their association with growth traits. Molecular and Cellular Probes, 28 (4): 200–203.
Yu L Z, Tang H, Wang J Y, Wu Y, Zou L L, Jiang Y L, Wu C X, Li N. 2007. Polymorphisms in the 5' regulatory region of myostatin gene are associated with early growth traits in Yorkshire pigs. Science in China Series C: Life Sciences, 50 (5): 642–647.
Zhang G X, Zhao X H, Wang J Y, Ding F X, Zhang L. 2011. Effect of an exon 1 mutation in the myostatin gene on the growth traits of the Bian chicken. Anim. Genet., 43 (4): 458–459.
Acknowledgement
We thank W. LI for assisting with sample preparation.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Supported by the National Science Foundation for Young Scientists of China (No. 31402296), the National Science Foundation for Post-Doctoral Scientists of China (No. 2014M562077), and the Wuhan Chenguang Project for Young Scholars (No. 201372304010832)
Rights and permissions
About this article
Cite this article
Sun, Y., Li, Q., Wang, G. et al. Polymorphisms in the Myostatin-1 gene and their association with growth traits in Ancherythroculter nigrocauda . Chin. J. Ocean. Limnol. 35, 597–602 (2017). https://doi.org/10.1007/s00343-017-5317-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00343-017-5317-0