Abstract
The industrially important organic compound 1,3-propanediol (1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K2 HPO4, 7.6 g/L (NH4)2 SO4, 3.0 g/L KH2 PO4, pH 7.1, cultivation at 35°C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L·h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.
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Supported by the Scientific Research Project of Marine Public Welfare Industry of China (No. 201205020-4), the Knowledge Innovation Project of Chinese Academy of Sciences (No. KSCX2-EW-G-12B), and the Administration of Ocean and Fisheries of Guangdong Province (No. GD2012-D01-002)
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Li, L., Zhou, S., Ji, H. et al. Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology. Chin. J. Ocean. Limnol. 32, 1036–1045 (2014). https://doi.org/10.1007/s00343-014-3267-3
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DOI: https://doi.org/10.1007/s00343-014-3267-3