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Methods for vascularization and perfusion of tissue organoids

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Abstract

Tissue organoids or “mini organs” can be invaluable tools for understanding health and disease biology, modeling tissue dynamics, or screening potential drug candidates. Effective vascularization of these models is critical for truly representing the in vivo tissue environment. Not only is the formation of a vascular network, and ultimately a microcirculation, essential for proper distribution and exchange of oxygen and nutrients throughout larger organoids, but vascular cells dynamically communicate with other cells to modulate overall tissue behavior. Additionally, interstitial fluid flow, mediated by a perfused microvasculature, can have profound influences on tissue biology. Thus, a truly functionally and biologically relevant organoid requires a vasculature. Here, we review existing strategies for fabricating and incorporating vascular elements and perfusion within tissue organoids.

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Acknowledgements

None noted.

Funding

Funding was provided by National Institutes of Health (Grant no. R01AR069297) and National Science Foundation (Grant no. 1842675).

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  1. Advanced Solutions Life Sciences, 500 N Commercial St., Manchester, NH, 03101, USA

    Hannah A. Strobel, Sarah M. Moss & James B. Hoying

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  1. Hannah A. Strobel
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  2. Sarah M. Moss
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  3. James B. Hoying
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Correspondence to James B. Hoying.

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JBH is a partner at Advanced Solutions Life Sciences, which commercializes vascularization technology. HAS and SMM are employees of ASLS.

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Strobel, H.A., Moss, S.M. & Hoying, J.B. Methods for vascularization and perfusion of tissue organoids. Mamm Genome 33, 437–450 (2022). https://doi.org/10.1007/s00335-022-09951-2

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