Quantitative trait locus and haplotype mapping in closely related inbred strains identifies a locus for open field behavior
- 225 Downloads
Quantitative trait locus (QTL) mapping in the mouse typically utilizes inbred strains that exhibit significant genetic and phenotypic diversity. The development of dense SNP panels in a large number of inbred strains has eliminated the need to maximize genetic diversity in QTL studies as plenty of SNP markers are now available for almost any combination of strains. We conducted a QTL mapping experiment using both a backcross (N2) and an intercross (F2) between two genetically similar inbred mouse strains: C57BL/6J (B6) and C57L/J (C57). A set of additive QTLs for activity behaviors was identified on Chrs 1, 9, 13, and 15. We also identified additive QTLs for anxiety-related behaviors on Chrs 7, 9, and 16. A QTL on Chr 11 is sex-specific, and we revealed pairwise interactions between QTLs on Chrs 1 and 13 and Chrs 10 and 18. The Chr 9 activity QTL accounts for the largest amount of phenotypic variance and was not present in our recent analysis of a B6 × C58/J (C58) intercross (Bailey et al. in Genes Brain Behav 7:761–769, 2008). To narrow this QTL interval, we used a dense SNP haplotype map with over 7 million real and imputed SNP markers across 74 inbred mouse strains (Szatkiewicz et al. in Mamm Genome 19(3):199–208, 2008). Evaluation of shared and divergent haplotype blocks among B6, C57, and C58 strains narrowed the Chr 9 QTL interval considerably and highlights the utility of QTL mapping in closely related inbred strains.
- Alttoa A, Eller M, Herm L, Rinken A, Harro J (2007) Amphetamine-induced locomotion, behavioral sensitization to amphetamine, and striatal D2 receptor function in rats with high or low spontaneous exploratory activity: differences in the role of locus coeruleus. Brain Res 1131:138–148CrossRefPubMedGoogle Scholar
- Beavis W (1994) The power and deceit of QTL experiments: lessons from comparative QTL studies. In: 49th Annual corn and sorghum research conference. American Seed Trade Association, Washington, DC, pp 252-268 Google Scholar
- Laarakker MC, Ohl F, van Lith HA (2008) Chromosomal assignment of quantitative trait loci influencing modified hole board behavior in laboratory mice using consomic strains, with special reference to anxiety-related behavior and mouse chromosome 19. Behav Genet 38:159–184CrossRefPubMedGoogle Scholar
- Russell E (1978) Genetic origins and some research uses of C57BL/6, DBA/2 and B6D2F1 mice. In: Gibson D, Adelman R, Finch C (eds) Development of the rodent as a model system of aging. USPHS-DHEW Publ (NIH), Washington, DC, pp 79–161Google Scholar