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Assessment of diabetic peripheral neuropathy in streptozotocin-induced diabetic rats with magnetic resonance imaging

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Abstract

Objective

To determine the role of magnetic resonance (MR) imaging and quantitative T2 value measurements in the assessment of diabetic peripheral neuropathy (DPN).

Methods

Sequential MR imaging, T2 measurement, and quantitative sensory testing of sciatic nerves were performed in streptozotocin-induced diabetic rats (n = 6) and normal control rats (n = 6) over a 7-week follow-up period. Histological assessment was obtained from 48 diabetic rats and 48 control rats once weekly for 7 weeks (n = 6 for each group at each time point). Nerve signal abnormalities were observed, and the T2 values, mechanical withdrawal threshold (MWT), and histological changes were measured and compared between diabetic and control animals.

Results

Sciatic nerves in the diabetic rats showed a gradual increase in T2 values beginning at 2 weeks after the induction (P = 0.014), while a decrease in MWT started at 3 weeks after the induction (P = 0.001). Nerve T2 values had a similar time course to sensory functional deficit in diabetic rats. Histologically, sciatic nerves of diabetic rats demonstrated obvious endoneural oedema from 2 to 3 weeks after the induction, followed by progressive axonal degeneration, Schwann cell proliferation, and coexistent disarranged nerve regeneration.

Conclusion

Nerve T2 measurement is potentially useful in detecting and monitoring diabetic neuropathy.

Key points

• Sciatic nerves in diabetic rats showed a gradual increase in T2 values

• Nerve T2 values were negatively correlated with sensory function impairment

• Longitudinal T2 values can be used to monitor the disease progress

• Nerve degeneration contributed mainly to progressive prolongation of nerve T2 values

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Acknowledgments

The scientific guarantor of this publication is Jun Shen. The authors of this manuscript declare no relationships with any companies whose products or services may be related to the subject matter of the article. This study has received funding from the National Natural Science Foundation of China (Grant number: 81371607) and the Fundamental Research Funds for the Central Universities of China (Grant number: 11ykzd13). No complex statistical methods were necessary for this paper. Institutional Review Board approval was obtained. Approval from the Institutional Animal Care Committee was obtained. Methodology: prospective, experimental, performed at one institution.

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Authors and Affiliations

  1. Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong, 51012, China

    Dongye Wang, Xiang Zhang, Liejing Lu, Haojiang Li, Fang Zhang, Yueyao Chen & Jun Shen

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  1. Dongye Wang
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  2. Xiang Zhang
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  3. Liejing Lu
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  5. Fang Zhang
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  7. Jun Shen
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Correspondence to Jun Shen.

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Wang, D., Zhang, X., Lu, L. et al. Assessment of diabetic peripheral neuropathy in streptozotocin-induced diabetic rats with magnetic resonance imaging. Eur Radiol 25, 463–471 (2015). https://doi.org/10.1007/s00330-014-3423-3

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  • DOI: https://doi.org/10.1007/s00330-014-3423-3

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