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Microbubbles assist goat liver ablation by high intensity focused ultrasound

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Abstract

High intensity focused ultrasound (HIFU) has been introduced to treat cancers. However, this therapy is a time-consuming procedure; destructing a deeper volume is also difficult as ultrasonic energy attenuates exponentially with increasing depth in tissues. The aim of the present study was to investigate the effects of introducing microbubbles on liver HIFU ablation. Seventeen goats were divided into groups A (n=8) and B (n=9). The livers in both groups were ablated using HIFU (1.0 MHz, 22,593 W/cm2) performed in the manner of a clinical regime using a clinical device. A microbubble agent was bolus-injected intravenously before HIFU exposure in group B. All animals in group A and seven goats in group B were euthanased to evaluate the ablation efficiency 24 h after HIFU. The necrosis rate (mm3/s), which was the volume of necrosis tissue per second of HIFU exposure, was used to judge the ablation efficiency. Pathological examinations were performed to determine whether there were residual intact tissues within the exposed volume. The other two goats in group B were used to determine the delayed pathological changes 7 days after ultrasonic ablation. The necrosis rate (mm3/s) was increased in group B (14.4647±4.1960 versus 33.5302±12.4484, P=0.0059). Pathological examinations confirmed that there were no residual unaffected tissue focuses within the exposed volume. Two remarkable changes occurred in the other two goats in group B 7 days after HIFU: there were ghost-cell islands at the periphery of the ablated tissues, and surrounding adjacent tissues outside the reactive zone necrotized. These findings showed that microbubbles could be used to assist liver HIFU ablation.

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Acknowledgements

This work was funded with grants from State Ministry of Education (NCET) and Chongqing Medical University (CX 200314).

We express our sincere thanks to Ms. Tao Cheng for helping preparing the Figs. 1, 2.

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Authors and Affiliations

  1. Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Tinghe Yu, Kai Hu & Zhibiao Wang

  2. Department of Microbiology, Huazhong University of Science and Technology, Tongji Medical School, Wuhan, 430030, People’s Republic of China

    Xionglin Fan

  3. Department of Gynecological Oncology, Jiangxi Provincial Maternal and Children’s Hospital, Nanchang, 330006, People’s Republic of China

    Shuhua Xiong

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  1. Tinghe Yu
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  2. Xionglin Fan
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Correspondence to Tinghe Yu.

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Yu, T., Fan, X., Xiong, S. et al. Microbubbles assist goat liver ablation by high intensity focused ultrasound. Eur Radiol 16, 1557–1563 (2006). https://doi.org/10.1007/s00330-006-0176-7

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  • DOI: https://doi.org/10.1007/s00330-006-0176-7

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