Abstract
Key message
Transgenic sugarcane overexpressing BRK1 showed improved tolerance to drought stress through modulation of actin polymerization and formation of interlocking marginal lobes in epidermal leaf cells, a typical feature associated with BRK1 expression under drought stress.
Abstract
BRICK1 (BRK1) genes promote leaf epidermal cell morphogenesis and division in plants that involves local actin polymerization. Although the changes in actin filament organization during drought have been reported, the role of BRK in stress tolerance remains unknown. In our previous work, the drought-tolerant Erianthus arundinaceus exhibited high levels of the BRK gene expression under drought stress. Therefore, in the present study, the drought-responsive gene, BRK1 from Saccharum spontaneum, was transformed into sugarcane to test if it conferred drought tolerance in the commercial sugarcane cultivar Co 86032. The transgenic lines were subjected to drought stress, and analyzed using physiological parameters for drought stress. The drought-induced BRK1-overexpressing lines of sugarcane exhibited significantly higher transgene expression compared with the wild-type control and also showed improved physiological parameters. In addition, the formation of interlocking marginal lobes in the epidermal leaf cells, a typical feature associated with BRK1 expression, was observed in all transgenic BRK1 lines during drought stress. This is the first report to suggest that BRK1 plays a role in sugarcane acclimation to drought stress and may prove to be a potential candidate in genetic engineering of plants for enhanced biomass production under drought stress conditions.
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Acknowledgements
The authors thank the Director, ICAR-Sugarcane Breeding Institute, Coimbatore, Tamil Nadu, India, for providing the facilities. The authors would like to thank Dr. Sheshakumar Goroshi, Scientist-E, Agro-Advisory Service Department, India Meteorological Department, MoES, New Delhi, for the statistical analysis of the data. The authors also wish to thank Mr. Brendan Crumrine, University of Florida, for English corrections. One of the authors, Ashwin Narayan J, thanks Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the award of Senior Research Fellowship (SRF) (09/706/0003/2018-EMR-I). This work was supported by the Department of Biotechnology (DBT) (Grant no. F. No. BT/PR12949/AGII/106/986/2015), Government of India, New Delhi. The authors would also thank Dr. Anilkumar C, Scientist, NRRI, Cuttack for his help in editing the manuscript and Mr. K. Selvamuthu for his assistance during the course of research for the maintenance of plants.
Funding
This study was funded by the Department of Biotechnology (DBT) (Grant no. F. No. BT/PR12949/AGII/106/986/2015), Government of India, New Delhi. The authors would also thank Dr. One of the authors, Ashwin Narayan J, thanks Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the award of Senior Research Fellowship (SRF) (09/706/0003/2018-EMR-I).
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All authors contributed to the study conception and design. JAN has performed the experimental work, and data analysis. MC and GN: repeated the experiments and observations. VMM and SD: assisted in analyzing data and gene expression validation studies. RV, RA, RG, and KKS: assisted in experiments, sampling, and physiological studies. JAN and GN: wrote the manuscript. NS and MNP: conceptualized, planned and reviewed the manuscript. GH: provided resources and helped in writing and review of manuscript. CA: conceptualization, investigation, data curation, project administration, supervision, visualization, writing—review and editing. All authors read and approved the final manuscript.
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Narayan, J.A., Manoj, V.M., Nerkar, G. et al. Transgenic sugarcane with higher levels of BRK1 showed improved drought tolerance. Plant Cell Rep 42, 1611–1628 (2023). https://doi.org/10.1007/s00299-023-03056-z
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DOI: https://doi.org/10.1007/s00299-023-03056-z