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Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation

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Abstract

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Abscisic acid induced the expression of AsKIN during the recovery period of garlic cryopreservation. AsKIN was identified as a gene involved in cold and osmotic stress resistance.

Cryopreservation has been proven to be effective in removing viruses from garlic. However, oxidative damage in cryopreservation has a significant impact on the survival after preservation. Abscisic acid (ABA) has been shown to reduce oxidative stress and promote the survival after cryopreservation. However, it is not clear which genes play important roles in this process. In this study, we added ABA to the dehydration step and analyzed the transcriptomic divergences between the ABA-treated group and the control group in three cryogenic steps (dehydration, unloading and recovery). By short time-series expression miner (STEM) analysis and weighted gene co-expression network analysis (WGCNA), the recovery step was identified as the period of significant changes in gene expression levels in cryopreservation. The addition of ABA promoted the upregulated expression of microtubule-related genes in the recovery step. We further identified AsKIN as a hub gene in the recovery step and verified its function. The results showed that overexpression of AsKIN enhanced the tolerance of Arabidopsis to oxidative stress in cryopreservation, influenced the expression of genes in response to cold and osmotic stress and promoted plant growth after stress. The AsKIN gene is likely to be involved in the plant response to cold stress and osmotic stress. These results reveal the molecular mechanisms of ABA in cryopreservation and elucidate the potential biological functions of the kinesin-14 subfamily.

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All data supporting the findings of this study are available within the paper and within its supplementary materials published online.

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Acknowledgements

The research was supported by National Natural Science Foundation of China (31372056; 31872125), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Fundamental Research Funds for the Central Universities (KJFP201702).

Funding

This study was funded by National Natural Science Foundation of China (31372056; 31872125), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Fundamental Research Funds for the Central Universities (KJFP201702).

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China

    Xiaodong Xing, Min Liu, Fangling Jiang, Rong Zhou, Yunhe Bai, Hanyu Wei, Deng Zhang, Jingjing Wei & Zhen Wu

  2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, Nanjing, China

    Xiaodong Xing, Min Liu, Fangling Jiang, Rong Zhou, Yunhe Bai, Hanyu Wei, Deng Zhang, Jingjing Wei & Zhen Wu

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ZW and XDX initiated and designed the research; XDX, YHB, HYW, DZ and JJW performed the experiments; XDX analyzed the data; ML contributed reagents/materials/analysis tools; XDX wrote the paper; ZW, FLJ and RZ revised the paper. All authors read and approved the final manuscript.

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Xing, X., Liu, M., Jiang, F. et al. Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation. Plant Cell Rep 41, 1955–1973 (2022). https://doi.org/10.1007/s00299-022-02894-7

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