Abstract
Key message
PePIP2;7, a leaf-specific aquaporin gene in bamboo, is upregulated under abiotic stresses. Overexpressing PePIP2;7 confers abiotic stresses tolerance in transgenic Arabidopsis plant and yeast.
Abstract
Aquaporins (AQPs) participate in the regulation of water balance in plants. However, the function of AQPs in bamboo remains unclear. Here, PePIP2;7 was identified as a leaf-specific aquaporin gene in moso bamboo based on the expression analysis of transcriptome data and PCR. In situ hybridization further indicated that PePIP2;7 was mainly expressed in mesophyll cells of mature leaves, while in immature leaves it was dominant in blade edge cells followed by mesophyll cells. Interestingly, PePIP2;7 was strongly expressed in the mesophyll cells near bulliform cells of immature leaves, suggesting that PePIP2;7 might function in water transport and contribute to leaf unfolding. The transient expression assay showed that PePIP2;7 was a plasma membrane intrinsic protein. Furthermore, PePIP2;7 was upregulated under abiotic stresses such as high light, drought, and NaCl. Compared with Col-0, transgenic Arabidopsis plants overexpressing PePIP2;7 had better seed germination rate, longer taproot length, higher SOD activity, and lower MDA content under abiotic stresses. Besides, yeasts expressing PePIP2;7 also had higher tolerance to stress compared to the control. Taken together, our results show that PePIP2;7 is leaf-specific and involved in stress response, which provides new insights into aquaporin function in bamboo.
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Abbreviations
- AQP:
-
Aquaporin
- bp:
-
Base pair
- CAT:
-
Catalase
- cDNA:
-
DNA complementary to RNA
- CDS:
-
Coding sequence
- dNTP:
-
Deoxyribonucleoside triphosphate
- F v/F m :
-
Maximum quantum yield in photosystem II
- GIP:
-
GlpF-like intrinsic protein
- HIP:
-
Hybrid intrinsic protein
- kDa:
-
Kilodalton
- MDA:
-
Malonaldehyde
- MW:
-
Molecular weight
- NPQ:
-
Non-photochemical quenching
- PIP:
-
Plasma membrane intrinsic protein
- POD:
-
Peroxidase
- NIP:
-
Nodulin 26-like intrinsic protein
- ORF:
-
Open reading frame
- pI:
-
Isoelectric point
- qRT-PCR:
-
Quantitative real-time PCR
- SIP:
-
Small basic intrinsic protein
- SOD:
-
Superoxide dismutase
- TIP:
-
Tonoplast intrinsic protein
- TM:
-
Transmembrane
- XIP:
-
Uncategorized X intrinsic protein
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Acknowledgements
The work was supported by the National Science Foundation of China (Grant nos. 31971736; 31930075) and the Special Funds for Fundamental Scientific Research on Professional Work Supported by the International Center for Bamboo and Rattan (No. 1632018005).
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ZMG and HYS designed the experiments; HYS and SNW performed experiments; HYS, SNW, LYF, and ZMG analyzed data; YFL, CLZ, HSZ, YL, and XPL contributed reagents/materials/analysis tools; HYS and ZMG wrote the paper. All authors read and approved the final manuscript. HS and SW contributed equally to this work.
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Sun, H., Wang, S., Lou, Y. et al. A bamboo leaf-specific aquaporin gene PePIP2;7 is involved in abiotic stress response. Plant Cell Rep 40, 1101–1114 (2021). https://doi.org/10.1007/s00299-021-02673-w
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DOI: https://doi.org/10.1007/s00299-021-02673-w