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Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco

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Abstract

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The 2-kb ZmCI - 1B promoter is active in the root and embryo and induced by wounding in maize and the 220-bp 5′-deleted segment maybe the minimal promoter.

Abstract

The subtilisin–chymotrypsin inhibitor gene, CI-1B of Zea mays (ZmCI-1B), has been suggested to induce the maize defense system to resist insect attack. Real-time RT-PCR showed that ZmCI-1B gene exhibited especially high expression in roots and embryos. The 2-kb full-length promoter of ZmCI-1B gene was isolated from the maize genome and used to drive expression of a beta-glucuronidase (GUS) reporter gene for transient expression and stable expression analysis in maize. The results of GUS histochemical staining in transgenic maize plants revealed that the ZmCI-1B promoter induced GUS expression preferentially in roots and embryos and in response to wounding. A series of 5′-deleted segments of the ZmCI-1B promoter were cloned individually to drive GUS expression for further analysis. Deletion analysis combined with the histochemical staining of transgenic tobacco plants revealed 220-bp segment could drive GUS in a tissue-specific and wounding-induced expression in tobacco; thus, it maybe the minimally active promoter of ZmCI-1B gene. Furthermore, it revealed that the ZmCI-1B promoter contained tissue-specific and wounding-induced elements.

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Abbreviations

CaMV:

Cauliflower mosaic virus

DAP:

Days after pollination

DAG:

Days after germination

ppt:

Phosphinothricin

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Acknowledgments

This study was supported by the National Special Program for GMO Development of China (grant number 2013ZX08003-002).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Crop Genomics and Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 ZhongGuanCun South Street, Beijing, 100081, China

    Ye Li, Xiaoqing Liu, Xiaojin Zhou, Wenzhu Yang & Rumei Chen

  2. College of Life Science, Beijing Normal University, 19 XinJieKouWai Avenue, Beijing, 100875, China

    Jie Li

  3. Department of Agronomy, Agricultural University of Hebei, 289 LingYuSi Avenue, Baoding, 071001, China

    Suzhen Li

  4. High School Affiliated to Chinese Renmin University, 37 ZhongGuan Cun Street, Beijing, 100080, China

    Guanyu Chen

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  1. Ye Li
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Correspondence to Rumei Chen.

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Communicated by J. S. Shin.

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Li, Y., Liu, X., Li, J. et al. Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco. Plant Cell Rep 34, 1443–1457 (2015). https://doi.org/10.1007/s00299-015-1799-4

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