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Peanut violaxanthin de-epoxidase alleviates the sensitivity of PSII photoinhibition to heat and high irradiance stress in transgenic tobacco

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Abstract

Key Message

This is the first study on peanut VDE, which led to multiple biochemical and physiological changes to heat and HI stress by improving de-epoxidation of the xanthophylls cycle.

Abstract

A peanut (Arachis hypogaea L.) violaxanthin de-epoxidase gene (AhVDE) was isolated by RT-PCR and RACE methods. The deduced amino acid sequence of AhVDE showed high identities with violaxanthin de-epoxidase of other plant species. The expression of AhVDE was obviously upregulated by 4, 40 °C and high light, NaCl, and abscisic acid. Sense and RNAi transgenic tobaccos were further used to investigate the physiological effects and functional mechanism of AhVDE. Compared with WT, the content of Z, the ratio of (A + Z)/(V + A + Z) and the non-photochemical quenching were higher in sense plants, and lower in the RNAi lines under heat and high irradiance (HI) stress, respectively. Additionally, photoinhibition of photosystem II (PSII) reflected by the maximal photochemical efficiency in WT lines was more severe, and in the RNAi lines was the most severe compared with that in the sense lines. Meanwhile, overexpressing AhVDE also led to multiple biochemical and physiological changes under heat and HI stress. Higher activities of superoxide dismutase and ascorbate peroxidase, lower content of reactive oxygen species and slighter membrane damage were observed in sense lines after heat and HI stress. These results suggested that, peanut VDE can alleviate PSII photoinhibition to heat and HI stress by improving the xanthophyll cycle-dependent energy dissipation.

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Abbreviations

A:

Antheraxanthin

ABA:

Abscisic acid

APX:

Ascorbate peroxidase

DAB:

3,3-diaminobenzidine

Fv/Fm:

The maximal photochemical efficiency of PSII

MDA:

Measurement of malondialdehyde

MGDG:

Monogalactosyldiacylglycerol

MS:

Murashige and Skoog

NBT:

Nitroblue tetrazolium

NPQ:

Nonphotochemical quenching

PSII:

Photosystem II

REC:

Relative electric conductivity

ROS:

Reactive oxygen species

RT-PCR:

Reverse-transcription polymerase chain reaction

SD:

Standard deviation

SOD:

Superoxide dismutase

V:

Violaxanthin

VDE:

Violaxanthin de-epoxidase

Z:

Zeaxanthin

ZE:

Zeaxanthin epoxidase

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Acknowledgments

This work was supported by the Project of Significant Application of Agricultural Technology Innovation in Shandong Province, International Science and Technology Cooperation Program of China (2015DFA31190), the Supporting Plan of National Science and Technology of China (2014BAD11B04) and the earmarked fund for Modern Agro-industry Technology Research System (CARS-14) and Shandong Major Projects of Independent Innovation Achievement Transformation (2012ZHZXIA0418).

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The authors declare that we have no conflict of interest.

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Authors and Affiliations

  1. Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Sha Yang, De-Yun Meng, Lin-Lin Hou, Yan Li, Feng Guo, Jing-Jing Meng & Xin-Guo Li

  2. Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, 250100, China

    Shu-Bo Wan

  3. College of Life Sciences, Shandong University, Jinan, 250100, China

    De-Yun Meng

  4. Agronomy and Plant Protection College, QingDao Agricultural University, Qingdao, 266109, China

    Lin-Lin Hou

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  1. Sha Yang
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Correspondence to Xin-Guo Li.

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Communicated by L. Peña.

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Yang, S., Meng, DY., Hou, LL. et al. Peanut violaxanthin de-epoxidase alleviates the sensitivity of PSII photoinhibition to heat and high irradiance stress in transgenic tobacco. Plant Cell Rep 34, 1417–1428 (2015). https://doi.org/10.1007/s00299-015-1797-6

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