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A wheat aminocyclopropane-1-carboxylate oxidase gene, TaACO1, negatively regulates salinity stress in Arabidopsis thaliana

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Abstract

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TaACO1 could catalyze ACC into ethylene in vitro. Constitutive expression of TaACO1 in Arabidopsis conferred salt sensitivity, and TaACO1 regulates salt stress mainly via the DREB1/CBF signal transduction pathway.

Abstract

Ethylene signaling plays essential roles in mediating plant responses to biotic and abiotic stresses, besides regulating plant growth and development. The roles of ethylene biosynthesis in abiotic stress, however, remain elusive. In this study, an aminocyclopropane-1-carboxylate oxidase gene, TaACO1, affecting the terminal step in ethylene biosynthesis, was isolated from a salt-tolerant bread wheat introgression line Shanrong No. 3 (SR3) and its effect on salt-stress response was examined. Purified recombinant protein of TaACO1 heterogenously expressed in Escherchia coli could catalyze ACC into ethylene in vitro. TaACO1 transcripts were down-regulated by salt, drought, oxidative stress and ABA. TaACO1-transgenic plants conferred salt sensitivity as judged from the seed germination, cotyledon greening and the relative root growth under salt stress. Constitutive expression of TaACO1 in Arabidopsis increased AtMYB15 expression and suppressed the expression of stress-responsive genes AtRAB18, AtCBF1 and AtCBF3. These findings are helpful in understanding the roles of ethylene biosynthesis in plant salt-stress response.

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Acknowledgments

We thank Interactional Wheat Genome Sequencing Consortium for our access to blast wheat sequences. This research was financially supported by Major Program of Natural Science Foundation of China (31030053), National Transgenic Project (2011ZX08002-002), Research 973 Program of China (2009CB118300), Independent Innovation Foundation of Shandong University (2009GN034) and Scientific Research Award Foundation for Excellent Youth Scientists of Shandong Province (2012BSE27117). The GenBank accession number for TaACO1 is AGT79999.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, 250100, People’s Republic of China

    Donghua Chen, Xiaoyan Ma, Chunlong Li, Wei Zhang, Guangmin Xia & Mei Wang

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  1. Donghua Chen
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  2. Xiaoyan Ma
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  3. Chunlong Li
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  4. Wei Zhang
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  5. Guangmin Xia
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Correspondence to Mei Wang.

Additional information

Communicated by Renate Schmidt.

D. Chen and X. Ma contributed equally to this work.

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Chen, D., Ma, X., Li, C. et al. A wheat aminocyclopropane-1-carboxylate oxidase gene, TaACO1, negatively regulates salinity stress in Arabidopsis thaliana . Plant Cell Rep 33, 1815–1827 (2014). https://doi.org/10.1007/s00299-014-1659-7

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