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The TCP transcription factor PeTCP10 modulates salt tolerance in transgenic Arabidopsis

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Abstract

Key message

PeTCP10 can be induced by salt stresses and play important regulation roles in salt stresses response in transgenic Arabidopsis.

Abstract

Salt stress is one of the major adverse environmental factors that affect normal plant development and growth. PeTCP10, a Class I TCP member, was markedly expressed in moso bamboo mature leaf, root and stem under normal conditions and also induced by salt stress. Overexpressed PeTCP10 was found to enhance salt tolerance of transgenic Arabidopsis at the vegetative growth stage. It was also found capable to increase relative water content, while decreasing relative electrolyte leakage and Na+ accumulation of transgenic Arabidopsis versus wild-type (WT) plants at high-salt conditions. In addition, it improved antioxidant capacity of transgenic Arabidopsis plants by promoting catalase activity and enhanced their H2O2 tolerance. In contrast to WT plants, transcriptome analysis demonstrated that multiple genes related to abscisic acid, salt and H2O2 response were induced after NaCl treatment in transgenic plants. Meanwhile, overexpressed PeTCP10 improved the tolerance of abscisic acid. Moreover, luciferase reporter assay results showed that PeTCP10 is able to directly activate the expression of BT2 in transgenic plants. In contrary, the germination rates of transgenic plants were significantly lower than those of WT plants under high-NaCl conditions. Both primary root length and survival rate at the seedling stage are also found lower in transgenic plants than in WT plants. It is concluded that overexpressed PeTCP10 enhances salt stress tolerance of transgenic plants at the vegetative growth stage, and it also improves salt sensitiveness in both germination and seedling stages. These research results will contribute to further understand the functions of TCPs in abiotic stress response.

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Abbreviations

MDA:

Malondialdehyde

REL:

Relative electrical leakage

qRT-PCR:

Quantitative real-time polymerase chain reaction

LUC:

Luciferase

CAT:

Catalase

TCP:

TEOSINTE BRANCHED 1, CYCLOIDEA and PROLIFERATING CELL FACTOR

FPKM:

Fragments per kilobase of exon model per Million mapped fragments

RNA-seq:

RNA sequencing

FW:

Fresh weight

SOS:

Salt overly sensitive

Y1H:

Yeast one-hybrid

ABA:

Abscisic acid

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31670672) and the 2021 Graduate Innovation Fund of Anhui Agricultural University (Grant No. 2021yjs-12).

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Authors and Affiliations

  1. Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, 230036, China

    Yuzeng Xu, Huanlong Liu, Yameng Gao, Rui Xiong, Min Wu, Kaimei Zhang & Yan Xiang

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Contributions

Experiments in this study were designed by XY, XYZ, and LHL. XYZ and LHL performed experiments. GYM and XR performed experimental guidance. WM and ZKM performed data analysis. XYZ drafted the manuscript, LHL provided advice on the manuscript. All authors edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yan Xiang.

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Supplementary Information

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Supplementary file1 (TIF 109 KB) FPKM values of eight selected stress-responsive genes.

Supplementary file2 (TIF 150 KB) Relative expression of eight selected stress-responsive genes using qRT-PCR method.

299_2021_2765_MOESM3_ESM.tif

Supplementary file3 (TIF 54 KB) Sketch map of BT2 upstream promoter regions (a) and expression levels of BT2 after salt stress treatment (b) (RNA-seq data, values of FPKM).

Supplementary file4 (XLSX 11 KB) Primers used in this study.

299_2021_2765_MOESM5_ESM.xlsx

Supplementary file5 (XLSX 12 KB) Identification of stress (salt, H2O2 and osmotic stress) responsive DEGs under 200 mM NaCl condition in PeTCP10 OE lines versus WT using RNA-seq method.

Supplementary file6 (XLSX 9 KB) Putative binding sites of Class I TCP in salt stress-responsive DEGs promoter regions.

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Xu, Y., Liu, H., Gao, Y. et al. The TCP transcription factor PeTCP10 modulates salt tolerance in transgenic Arabidopsis. Plant Cell Rep 40, 1971–1987 (2021). https://doi.org/10.1007/s00299-021-02765-7

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