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Disrupted actin dynamics trigger an increment in the reactive oxygen species levels in the Arabidopsis root under salt stress

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Abstract

Changes in actin dynamics represent the primary response of the plant cell to extracellular signaling. Recent studies have now revealed that actin remodeling is involved in abiotic stress tolerance in plants. In our current study, the relationship between the changes in actin dynamics and the reactive oxygen species (ROS) level at the initial stages of salt stress was investigated in the elongation zone of the Arabidopsis root tip. We found that a 200 mM NaCl treatment disrupted the dynamics of the actin filaments within 10 min and increased the ROS levels in the elongation zone cells of the Arabidopsis root tip. We further found that the NADPH oxidase activity inhibitor, diphenyleneiodonium, treatment blocked this ROS increase under salt stress conditions. The roles of actin dynamics and the NADPH oxidases in ROS generation were further analyzed using the actin-specific agents, latrunculin B (Lat-B) and jasplakinolide (Jasp), and mutants of Arabidopsis NADPH oxidase AtrbohC. Lat-B and Jasp promote actin depolymerization and polymerization, respectively, and both were found to enhance the ROS levels following NaCl treatment. However, this response was abolished in the atrbohC mutants. Our present results thus demonstrate that actin dynamics are involved in regulating the ROS level in Arabidopsis root under salt stress conditions.

Key message Salt stress disrupts the dynamics of the actin filaments in Arabidopsis in the short term which are involved in regulating the ROS levels that arise under salt stress conditions via the actions of the AtrbohC.

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Acknowledgments

We thank Dr. Elison B. Blancaflor (Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA) for providing the transgenic line expressing GFP-ABD2-GFP, and the Arabidopsis Biological Resource Center (Ohio State University, www.arabidopsis.org/abrc) for kindly providing the Arabidopsis mutant seeds. This study is funded by the National Natural Science Foundation of China (31170293; 31000652), and the Opening Project of the State Key Laboratory of Plant Physiology and Biochemistry (SKLPPBKF09005).

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  1. Guang Hui Chen

    Present address: Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

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  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, 271018, Shandong, China

    Shang Gang Liu, Dong Zi Zhu, Guang Hui Chen, Xin-Qi Gao & Xian Sheng Zhang

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Correspondence to Xin-Qi Gao.

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Communicated by K. Chong.

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Liu, S.G., Zhu, D.Z., Chen, G.H. et al. Disrupted actin dynamics trigger an increment in the reactive oxygen species levels in the Arabidopsis root under salt stress. Plant Cell Rep 31, 1219–1226 (2012). https://doi.org/10.1007/s00299-012-1242-z

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