Abstract
The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It consists of 1,441 bp, with an open reading frame of 834 bp, encoding a predicted polypeptide of 278 amino acids. Sequence similarity as well as exon and intron characteristics clearly suggested that FaNYE1 encoded an AtNYE1-like chloroplast protein. FaNYE1 could be strongly induced by dark treatment and natural senescence. FaNYE1, driven by a 1.5 kb upstream fragment of AtNYE1, could rescue the stay-green phenotype of nye1-1. Constitutive overexpression of FaNYE1 in Arabidopsis resulted in the whole spectrum of leaf yellowing phenotypes, the severity of which correlates with its transcript level. These results collectively indicate that FaNYE1 might play an important regulatory role in chlorophyll degradation during senescence in tall fescue, and therefore is a valuable gene for improving the green period or lawn color of turf grasses by genetic engineering.
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Alos E, Roca M, Iglesias DJ, Minguez-Mosquera MI, Damasceno CMB, Thannhauser TW, Rose JKC, Talon M, Cercos M (2008) An evaluation of the basis and consequences of a stay-green mutation in the navel negra citrus mutant using transcriptomic and proteomic profiling and metabolite analysis. Plant Physiol 147:1300–1315
Armstead I, Donnison I, Aubry S, Harper J, Hortensteiner S, James C, Mani J, Moffet M, Ougham H, Roberts L, Thomas A, Weeden N, Thomas H, King I (2006) From crop to model to crop: identifying the genetic basis of the staygreen mutation in the Lolium/Festuca forage and amenity grasses. New Phytol 172:592–597
Armstead I, Donnison I, Aubry S, Harper J, Hortensteiner S, James C, Mani J, Moffet M, Ougham H, Roberts L, Thomas A, Weeden N, Thomas H, King I (2007) Cross-species identification of Mendel’s/locus. Science 315:73–73
Barry CS (2009) The stay-green revolution: recent progress in deciphering the mechanisms of chlorophyll degradation in higher plants. Plant Sci 176:325–333
Barry CS, McQuinn RP, Chung MY, Besuden A, Giovannoni JJ (2008) Amino acid substitutions in homologs of the STAY-GREEN protein are responsible for the green-flesh and chlorophyll retainer mutations of tomato and pepper. Plant Physiol 147:179–187
Benedetti CE, Arruda P (2002) Altering the expression of the chlorophyllase gene ATHCOR1 in transgenic Arabidopsis caused changes in the chlorophyll-to-chlorophyllide ratio. Plant Physiol 128:1255–1263
Borovsky Y, Paran I (2008) Chlorophyll breakdown during pepper fruit ripening in the chlorophyll retainer mutation is impaired at the homolog of the senescence-inducible stay-green gene. Theor Appl Genet 117:235–240
Cao YJ, Wei Q, Liao Y, Song HL, Li X, Xiang CB, Kuai BK (2009) Ectopic overexpression of AtHDG11 in tall fescue resulted in enhanced tolerance to drought and salt stress. Plant Cell Rep 28:579–588
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
Hauck B, Gay AP, Macduff J, Griffiths CM, Thomas H (1997) Leaf senescence in a non-yellowing mutant of Festuca pratensis: implications of the stay-green mutation for photosynthesis, growth and nitrogen nutrition. Plant Cell Environ 20:1007–1018
Holsters M, de Waele D, Depicker A, Messens E, van Montagu M, Schell J (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163:181–187
Hortensteiner S (2006) Chlorophyll degradation during senescence. Annu Rev Plant Biol 57:55–77
Jiang HW, Li MR, Liang NB, Yan HB, Wei YL, Xu X, Liu JF, Xu Z, Chen F, Wu GJ (2007) Molecular cloning and function analysis of the stay green gene in rice. Plant J 52:197–209
Kingston Smith AH, Thomas H, Foyer CH (1997) Chlorophyll a fluorescence, enzyme and antioxidant analyses provide evidence for the operation of alternative electron sinks during leaf senescence in a stay-green mutant of Festuca pratensis. Plant Cell Environ 20:1323–1337
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25:402–408
Moore BJ, Donnison IS, Harper JA, Armstead IP, King J, Thomas H, Jones RN, Jones TH, Thomas HM, Morgan WG, Thomas A, Ougham HJ, Huang L, Fentem T, Roberts LA, King IP (2005) Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis. New Phytol 165:801–806
Mullan DJ, Colmer TD, Francki MG (2007) Arabidopsis–rice–wheat gene orthologues for Na+ transport and transcript analysis in wheat–L. elongatum aneuploids under salt stress. Mol Genet Genomics 277:199–212
Park SY, Yu JW, Park JS, Li J, Yoo SC, Lee NY, Lee SK, Jeong SW, Seo HS, Koh HJ, Jeon JS, Park YI, Paek NC (2007) The senescence-induced staygreen protein regulates chlorophyll degradation. Plant Cell 19:1649–1664
Ren G, An K, Liao Y, Zhou X, Cao Y, Zhao H, Ge X, Kuai B (2007) Identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence in Arabidopsis. Plant Physiol 144:1429–1441
Ren G, Zhou Q, Wu S, Zhang Y, Zhang L, Huang J, Sun Z, Kuai B (2010) Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in Arabidopsis. J Integr Plant Biol 52:496–504
Sato Y, Morita R, Nishimura M, Yamaguchi H, Kusaba M (2007) Mendel’s green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway. Proc Natl Acad Sci USA 104:14169–14174
Schelbert S, Aubry S, Burla B, Agne B, Kessler F, Krupinska K, Hortensteiner S (2009) Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis. Plant Cell 21:767–785
Vicentini F, Hortensteiner S, Schellenberg M, Thomas H, Matile P (1995) Chlorophyll breakdown in senescent leaves—identification of the biochemical lesion in a stay-green genotype of Festuca pratensis Huds. New Phytol 129:247–252
Wang ZY, Ge YX (2006) Invited review: recent advances in genetic transformation of forage and turf grasses. In Vitro Cell Dev Plant 42:1–18
Wang ZY, Hopkins A, Mian R (2001) Forage and turf grass biotechnology. Crit Rev Plant Sci 20:573–619
Wei Q, Guo Y, Cao H, Kuai B (2010) Cloning and characterization of an AtNHX2-like Na+/H+ antiporter gene from Ammopiptanthus mongolicus (Leguminosae) and its ectopic expression enhanced drought and salt tolerance in Arabidopsis thaliana. Plant Cell Tissue Organ Cult. doi:10.1007/s11240-010-9869-3
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This work was supported by a grant from the Natural Science Foundation of China (Grant no. 30770169), and a grant from the Science and Technology Committee of the Shanghai Municipal Government (Grant no. 08JC1400700).
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Communicated by E. Guiderdoni.
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Wei, Q., Guo, Y. & Kuai, B. Isolation and characterization of a chlorophyll degradation regulatory gene from tall fescue. Plant Cell Rep 30, 1201–1207 (2011). https://doi.org/10.1007/s00299-011-1028-8
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DOI: https://doi.org/10.1007/s00299-011-1028-8