Summary
The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10-7 transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10-7 transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10-8. These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce “opine” synthesis in Crown-gall plant cells.
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Holsters, M., de Waele, D., Depicker, A. et al. Transfection and transformation of Agrobacterium tumefaciens . Molec. Gen. Genet. 163, 181–187 (1978). https://doi.org/10.1007/BF00267408
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DOI: https://doi.org/10.1007/BF00267408