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Comparative characterization, expression pattern and function analysis of the 12-oxo-phytodienoic acid reductase gene family in rice

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Abstract

The 12-oxo-phytodienoic acid reductases (OPRs) belong to the old yellow enzyme family of flavoenzymes and form multiple subfamilies in angiosperm plants. In our previous study, a comparative genomic analysis showed that five OPR subfamilies (subs. I–V) occur in monocots, and two subfamilies (subs. I and II) in dicots. Here, a comparative study of five OsOPR genes, representing five subfamilies (I–V) in rice, was performed to provide insights into OPR biochemical properties and physiological importance. Comparative analysis of the three-dimensional structure by homology modeling indicated all five OsOPR proteins contained a highly conserved backbone with (α/β)8-barrels, while two middle variable regions (MVR i and ii) were also detected and defined. Analysis of enzymatic characteristics revealed that all five OsOPR fusion proteins exhibit distinct substrate specificity. Different catalytic activity was observed using racemic OPDA and trans-2-hexen-1-al as substrates, suggesting OsOPR family genes participate in two main branches of the octadecanoid pathway, including the allene oxide synthase and hydroperoxide lyase pathways which regulate various developmental processes and/or defense responses. The transcript profiles of five OsOPR genes exhibited strong tissue-specific and inducible expression patterns under abiotic stress, hormones and plant wounding treatments. Furthermore, the transcriptions of OsOPR04-1 (OsOPR11) and OsOPR08-1 (OsOPR7), representing subs. I and II, respectively, were observed in all six selected tissues and with all above-stress treatments. This suggests that these two subfamilies play an important role during different developmental stages and in response to stresses; while the expressions of OsOPR06-1 (OsOPR6), OsOPR01-1 (OsOPR10) and OsOPR02-1 (OsOPR8), representing subs. III, IV and V respectively, were strongly up-regulated with abscisic acid (ABA) and indoleacetic acid (IAA) treatments in roots, suggesting these three subfamilies play an important role in responding to hormones especially ABA and IAA signals in roots.

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (No. 30800600 and No. 30970237), the Natural Science Foundation of Guangdong Province, P R China (No. 8151027501000016) and the Fundamental Research Funds for the Central Universities (No. 10lgpy34).

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Authors and Affiliations

  1. State Key Laboratory for Biocontrol and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China

    Wenyan Li, Bing Liu, Dongru Feng, Yanming He, Kangbiao Qi, Hongbin Wang & Jinfa Wang

  2. College of Life Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Feng Zhou

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  1. Wenyan Li
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Correspondence to Hongbin Wang.

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Communicated by M. Jordan.

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Li, W., Zhou, F., Liu, B. et al. Comparative characterization, expression pattern and function analysis of the 12-oxo-phytodienoic acid reductase gene family in rice. Plant Cell Rep 30, 981–995 (2011). https://doi.org/10.1007/s00299-011-1002-5

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  • DOI: https://doi.org/10.1007/s00299-011-1002-5

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