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Relationship between Rh-RTH1 and ethylene receptor gene expression in response to ethylene in cut rose

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Abstract

A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6, 61.2, 42.5 and 39.8% identity with that of Arabidopsis RTH, RTE1, tomato GRL2, GRL1 and GR, respectively. Northern hybridization indicated that Rh-RTH1 expression is enhanced by endogenous and exogenous ethylene and inhibited by 1-MCP in petals and gynoecia. Rh-RTH1 expression partly correlated with sites of the ethylene receptor gene Rh-ETR1 and Rh-ETR3 expression, such as the petals, gynoecia, roots, and buds. The induction of Rh-RTH1 and Rh-ETR3 expression was substantially suppressed by 1-MCP treatment, while Rh-ETR1 expression was not reduced by 1-MCP treatment. Following treatment of flowers with sucrose, the level of Rh-RTH1 and Rh-ETR3 mRNA was only slightly decreased in petals and gynoecia. Upon wounding treatment, Rh-RTH1, Rh-ETR1 and Rh-ETR3 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The expression of Rh-RTH1 showed partial correlation with that of Rh-ETR1 and Rh-ETR3.

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Abbreviations

CTR:

Constitutive triple response

EIN:

Ethylene insensitive

ETR:

Ethylene resistant

ERS:

Ethylene response sensor

PCR:

Polymerase chain reaction

RACE:

Rapid-amplification of cDNA ends

RT:

Reverse transcription

RTH:

RTE1-HOMOLOG

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Acknowledgments

This study was supported by Fok Ying Tung Education Foundation (104031), and National Natural Science Foundation of China (30800758 and 30972410).

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Authors and Affiliations

  1. College of Horticulture, South China Agricultural University, Guangzhou, 510642, China

    Yixun Yu, Jing Wang, Huinan Wang, Zhaoqi Zhang & Juanxu Liu

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  1. Yixun Yu
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  2. Jing Wang
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  3. Huinan Wang
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  4. Zhaoqi Zhang
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  5. Juanxu Liu
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Correspondence to Juanxu Liu.

Additional information

Communicated by J. R. Liu.

Y. Yu and J. Wang contributed equally to this work.

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Yu, Y., Wang, J., Wang, H. et al. Relationship between Rh-RTH1 and ethylene receptor gene expression in response to ethylene in cut rose. Plant Cell Rep 29, 895–904 (2010). https://doi.org/10.1007/s00299-010-0875-z

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  • DOI: https://doi.org/10.1007/s00299-010-0875-z

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