Abstract
Ethylene receptor (ethylene response sensor, ERS) is the primary component involving in the ethylene biosynthesis and ethylene signal transduction pathway. In the present study, a GZ-ERS gene encoding ERS was cloned from a sugarcane cv. YL17 (Saccharum spp.) using RT-PCR and ligation-mediated PCR with primers designed based on conserved amino acid sequences of ERS from rice and corn. The GZ-ERS gene was 2,243 base pairs in length and coded for a polypeptide of 633 amino acid residues. The amino acid sequence of GZ-ERS encoded protein was 97, 96 and 91 % identical to OS-ERS (AAX95525) of rice, ZmERS25 (AAR25567) of corn, and Sc-ERS (ACF60981) of a sugarcane cv. ROC22, respectively. Genomic Southern analysis using a GZ-ERS cDNA insert as the probe indicated the presence of a single copy of GZ-ERS gene per haploid sugarcane genome. Real-time qPCR analysis showed that GZ-ERS was widely expressed in the vegetative tissues of sugarcane, and its expression in maturing internodes and leaf tissues was higher than in immature tissues. Furthermore, the expression level of GZ-ERS was the highest in maturing leaves, but very low in immature internodes. The GZ-ERS expression level in maturing leaves was enhanced by ethephon treatment, but was reduced under low temperature, drought, and dark conditions. The results from this study may help understand the regulation of gene expression in sugarcane and its responses to plant growth regulator ethephon and environmental stresses. As the sugarcane ERS family has multiple members, other members need to be isolated and characterized in future studies.
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Acknowledgments
The authors would like to thank Beijing Proteome Research Center, China for MALDI TOF/TOF MS analysis and Perng-Kuang Chang, Duli Zhao, and Zhanguo Xin for reviewing the manuscript. This work was supported by China National R & D Research Program Project (2007BAD30B00), International Scientific Exchange Program Projects (2008DFA30600, 2009DFA30820), Guangxi R & D Research Program Projects (Gui Ke Gong 0782004-3, Gui Ke Neng 0815011) and Guangxi Natural Science Foundation Projects (2010GXNSFA013101, 2011GXNSFF018002).
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Wang, AQ., Ye, XZ., Huang, JL. et al. Molecular Cloning and Functional Analysis of an Ethylene Receptor Gene from Sugarcane (Saccharum spp.) by Hormone and Environmental Stresses. Sugar Tech 17, 22–30 (2015). https://doi.org/10.1007/s12355-014-0324-3
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DOI: https://doi.org/10.1007/s12355-014-0324-3