Skip to main content
SpringerLink
Log in

Expression of an elicitor-encoding gene from Magnaporthe grisea enhances resistance against blast disease in transgenic rice

  • Genetic Transformation and Hybridization
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Elicitors are molecules that stimulate defense responses in plants. Previously, an elicitor-encoding gene, named pemG1, was isolated from Magnaporthe grisea. To assess the function of pemG1 in rice (Oryza sativa L. cv. Nipponbare), the gene was cloned under a constitutive maize ubiquitin promoter and introduced into Nipponbare cultivar. The resultant plants showed stable integration and constitutive expression of the pemG1 gene. The expression of defense-related gene for phenylalanine ammonia-lyase was triggered and proline content was also increased in pemG1-expressing plants. The pemG1-expressing plants showed enhanced resistance against rice blast after inoculation with M. grisea spores, suggesting that the pemG1 expression enhances disease resistance in transgenic rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Amero SA, James TC, Elgin SCR (2002) Production of antibodies using proteins in gel bands. In: Walker JM (ed) The protein protocols handbook, 2nd edn, Part VII edn. Humana Press, Totowa, pp 941–944

    Google Scholar 

  • Arlat M, Gijsegem FV, Huet JC, Pernollet JC, Doucher CA (1994) PopA1, a protein, which induces a hypersensitivelike response on specific petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum. EMBO J 13:43–553

    Google Scholar 

  • Baillieul F, Genetet I, Kopp MA, Saindrenan P, Fritig B, Kauffmann S (1995) A new elicitor of the hypersensitive response in tobacco: a fungal glycoprotein elicits cell death, expression of defense genes, production of salicylic acid, and induction of systemic acquired resistance. Plant J 8:551–560

    Article  CAS  PubMed  Google Scholar 

  • Boissy G, Fprtelle DL, Kahn R (1996) Crystal structure of a fungal elicitor secreted by Phytophthora cryptogea, a member of a novel class of plant necrotic protein. Structure 4:1429–1432

    Article  CAS  PubMed  Google Scholar 

  • Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledoneus plants. Transgenic Res 5:216–218

    Article  Google Scholar 

  • Coca M, Bortolotti C, Rufat M, Penas G, Eritja R, Tharreau D, Pozo AM, Messeguer J, Segundo BS (2004) Transgenic rice plants expressing the antifungal AFP protein from Aspergillus giganteus show enhanced resistance to the rice blast fungus Magnaporthe grisea. Plant Mol Biol 54:245–259

    Article  CAS  PubMed  Google Scholar 

  • Coca M, Peñas G, Gómez J, Campo S, Bortolotti C, Messeguer J, Segundo BS (2006) Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice. Planta 223:392–406

    Article  CAS  PubMed  Google Scholar 

  • Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097

    Article  CAS  PubMed  Google Scholar 

  • Fang LJ, Fu XQ, Ye QS, Wu T, Wang ZC, Chen X, Zhu ZK, Zhang XF, Fu CK (2007) Effects of p-glycoprotein inhibitor and elicitor on the salt tolerance of rice seedlings. J Integr Plant Biol 49:588–597

    Article  CAS  Google Scholar 

  • Gandikota M, Kochko A, Chen L, Ithal N, Fauquet C, Reddy AR (2001) Development of transgenic rice plants expressing maize anthocyanin genes and increased blast resistance. Mol Breed 7:73–83

    Article  CAS  Google Scholar 

  • Ger MJ, Chen CH, Wang SY, Huang HE, Podile AR, Dayakar BV, Feng TY (2002) Constitutive expression of hrap gene in transgenic tobacco plant enhances resistance against virulent bacterial pathogens by induction of a hypersensitive response. Mol Plant Microbe Interact 15:764–773

    Article  CAS  PubMed  Google Scholar 

  • Guo B, Liang YC, Li ZJ, Guo W (2007) Role of salicylic acid in alleviating cadmium toxicity in rice roots. J Plant Nutr 30:427–439

    Article  CAS  Google Scholar 

  • He SY, Huang HC, Collmer A (1993) Pseudomonas syringae pv. syringae Harpinpss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. Cell 73:1255–1266

    Article  CAS  PubMed  Google Scholar 

  • Keller H, Pamboukdjian N, Ponchet M, Poupet A, Delon R, Verrier JL, Roby D, Ricci P (1999) Pathogen-induced elicitin production in transgenic tobacco generates a hypersensitive response and nonspecific disease resistance. Plant Cell 11:223–235

    Article  CAS  PubMed  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–683

    Article  CAS  PubMed  Google Scholar 

  • Levine A, Tenhaken R, Dixon R, Lamb C (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79:583–593

    Article  CAS  PubMed  Google Scholar 

  • Maheshwari R, Dubey RS (2007) Nickel toxicity inhibits ribonuclease and protease activities in rice seedlings: protective effects of proline. Plant Growth Regul 51:231–243

    Article  CAS  Google Scholar 

  • Maruthasalam S, Kalpana K, Kumar KK, Loganathan M, Poovannan K, Raja JAJ, Kokiladevi E, Samiyappan R, Sudhakar D, Balasubramanian P (2007) Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight. Plant Cell Rep 26:791–804

    Article  CAS  PubMed  Google Scholar 

  • Moreno AB, Penas G, Rufat M, Bravo JM, Estopa M, Messeguer J, Segundo BS (2005) Pathogen-induced production of the antifungal AFP protein from Aspergillus giganteus confers resistance to the blast fungus Magnaporthe grisea in transgenic rice. Mol Plant-Microb Interact 18:960–972

    Article  CAS  Google Scholar 

  • Nespoulous C, Huet JC, Pernollet JC (1992) Strcture–function relationships of α and β elicitins, singal proteins involved in the plant–phytophthora interaction. Planta 186:551–557

    Article  CAS  Google Scholar 

  • Nishizawa Y, Nishio Z, Nakazono K, Soma M, Nakajima E, Ugaki M, Hibi T (1999) Enhanced resistance to blast (Magnaporthe grisea) in transgenic aponica rice by constitutive expression of rice chitinase. Theor Appl Genet 99:383–390

    Article  CAS  Google Scholar 

  • Nürnberger T, Nennstiel D, Jabs T, Sacks WR, Hahlbrock K, Scheel D (1994) High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses. Cell 78:449–460

    Article  PubMed  Google Scholar 

  • Patkar RN, Chattoo BB (2006) Transgenic indica rice expressing ns-LTP-like protein shows enhanced resistance to both fungal and bacterial pathogens. Mol Breed 17:159–171

    Article  CAS  Google Scholar 

  • Peng JL, Bao ZL, Ren HY, Wang JS, Dong HS (2004) Expression of harpin(Xoo) in transgenic tobacco induces pathogen defense in the absence of hypersensitive cell death. Phytopathology 94:1048–1055

    Article  CAS  PubMed  Google Scholar 

  • Pritilata N, Debabrata B, Sampa D, Asitava B, Dipankar G, Neeliyath A, Maloy G, Soumitra KS (1997) Transgenic elite indica rice plants expressing CryIAc ∂-endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas). PNAS 94:2111–2116

    Article  Google Scholar 

  • Ricci P (1997) Induction of the hypersensitive response and systemic acquired resistance by fungal proteins: the case of elicitins. In: Stacey G, Keen NT (eds) Plant–microbe interactions, vol 3. Chapman and Hall, New York

    Google Scholar 

  • Ricci P, Bonnet P, Huet JC, Sallantin M, Beauvais CF, Bruneteau M, Billard V, Michel G, Pernollet JC (1989) Structure and activity of proteins from pathogenic fungi Phytophthora eliciting necrosis and acquired resistance in tobacco. Eur J Biochem 183:555–563

    Article  CAS  PubMed  Google Scholar 

  • Saha P, Majumder P, Ray IDT, Roy SC, Das S (2006) Transgenic rice expressing Allium sativum leaf lectin with enhanced resistance against sap-sucking insect pests. Planta 223:1329–1343

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 931–944

    Google Scholar 

  • Shah K, Dubey RS (1998) Effect of cadmium on proline accumulation and ribonuclease activity in rice seedlings: role of proline as a possible enzyme protectant. Biol Plant 40:121–130

    Article  Google Scholar 

  • Shao M, Wang J, Dean RA, Lin Y, Gao X, Hu S (2008) Expression of a harpin-encoding gene in rice confers durable nonspecific resistance to Magnaporthe grisea. Plant Biotechnol J 6(1):73–81

    CAS  PubMed  Google Scholar 

  • Shigeru T, Mitsuo O, Yusuke J, Hisakazu Y, Hanae K, Naoto S, Eiichi M (2006) Induction of resistance against rice blast fungus in rice plants treated with a potent elicitor, N-acetylchitooligosaccharide. Biosci Biotechnol Biochem 70:1599–1605

    Article  Google Scholar 

  • Sorho F, Pinel A, Traoré O, Bersoult A, Ghesquière A, Hébrard E, Konaté G, Séré Y, Fargette D (2005) Durability of natural and transgenic resistances in rice to rice yellow mottle virus. Eur J Plant Pathol 112:349–359

    Article  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503

    Article  CAS  PubMed  Google Scholar 

  • Takakura Y, Che FS, Ishida Y, Tsutsumi F, Kurotani K, Usami S, Isogai A, Imaseki H (2008) Expression of a bacterial flagellin gene triggers plant immune responses and confers disease resistance in transgenic rice plants. Mol Plant Pathol 9(4):525–529

    Article  CAS  PubMed  Google Scholar 

  • Tang KX, Hu QA, Sun XF, Wan BL, Qi HX, Lu XG (2001) Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper. In Vitro Cell Dev Biol Plant 37:334–340

    Article  CAS  Google Scholar 

  • Toki S, Hara N, Ono K, Onodera H, Tagiri A, Oka S, Tanaka H (2006) Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice. Plant J 47:969–976

    Article  CAS  PubMed  Google Scholar 

  • Umemura K, Tanino S, Nagatsuka T, Koga J, Iwata M, Nagashima K, Amemiya Y (2004) Cerebroside elicitor confers resistance to Fusarium disease in various plant species. Phytopathology 94:813–818

    Article  CAS  PubMed  Google Scholar 

  • Veit S, Wörle JM, Nürnberger T, Koch W, Seitz HU (2001) A novel protein elicitor (PaNie) from pythium aphanidermatum induces multiple defense responses in carrot, Arabidopsis, and tobacco. Plant Physil 127:832–841

    Article  CAS  Google Scholar 

  • Wei ZM, Laby RJ, Zumoff CH, Bauer DW, He SY, Collmer A, Beer SV (1992) Harpin, elicitor of the hypersensitive response produced by the plant pathogen, Erwinia amylovora. Science 257:85–88

    Article  CAS  PubMed  Google Scholar 

  • Wei YP, Yao FY, Zhu CX, Jiang MS, Li GX, Song YZ, Wen FJ (2007) Breeding of transgenic rice restorer line for multiple resistance against bacterial blight, striped stem borer and herbicide. Biol plant 51:340–342

    Article  Google Scholar 

  • Xu F, Yang Y, Xie FJ, Liu Z, Qiu DW, Yang XF (2006) Effect of the activator protein from Magnaporthe grisea on plant growth and physiological activities. Acta Agriculturae Boreali-Sinica 21:1–5

    Google Scholar 

Download references

Acknowledgments

We thank Zachary Huang of the Michigan State University for editing assistance and Zhu Haiying at the University of Washington for technical assistance. This work was supported by grants from the Ministry of Science and Technology of China, project numbers 973 (2003CB114204) and 863 (2006AA10A210), and the Beijing Grand Project (D706005040431) as well.

Author information

Authors and Affiliations

  1. The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, 100081, Beijing, People’s Republic of China

    Dewen Qiu, Jianjun Mao, Xiufen Yang & Hongmei Zeng

Authors
  1. Dewen Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jianjun Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiufen Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hongmei Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dewen Qiu.

Additional information

Communicated by A. Feher.

DQ and JM contributed equally to this paper.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qiu, D., Mao, J., Yang, X. et al. Expression of an elicitor-encoding gene from Magnaporthe grisea enhances resistance against blast disease in transgenic rice. Plant Cell Rep 28, 925–933 (2009). https://doi.org/10.1007/s00299-009-0698-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-009-0698-y

Keywords

Search

Navigation