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Transgenic indica Rice Expressing ns-LTP-Like Protein Shows Enhanced Resistance to Both Fungal and Bacterial Pathogens

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Abstract

Antimicrobial peptides (AMPs) from plant seeds, known to inhibit pathogen growth have a great potential in developing transgenic plants resistant to disease. Some of the nonspecific-lipid transfer proteins (ns-LTP) that facilitate in vitro transport of lipids, show antimicrobial activity in vitro. Rice seeds also contain ns-LTPs; however, these genes are expressed weakly in seedlings. We have transformed Pusa Basmati 1, an elite indica rice cultivar, with the gene for Ace-AMP1 from Allium cepa, coding for an effective antimicrobial protein homologous to ns-LTPs. The gene for Ace-AMP1 was cloned under an inducible rice phenylalanine ammonia-lyase (PAL) or a constitutive maize ubiquitin (UbI) promoter. Ace-AMP1 was expressed in transgenic lines and secreted in the apoplastic space. Protein extracts from leaves of transgenic plants inhibited three major rice pathogens, Magnaporthe grisea, Rhizoctonia solani and Xanthomonas oryzae, in vitro. Enhanced resistance against these pathogens was observed in in planta assays, and the degree of resistance correlating with the levels of Ace-AMP1 with an average increase in resistance to blast, sheath blight, and bacterial leaf blight disease by 86%, 67%, and 82%, respectively. Importantly, transgenic rice plants, with stable integration and expression of Ace-AMP1, retained their agronomic characteristics while displaying enhanced resistance to both fungal and bacterial pathogens.

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References

  • Y. Bi B.P.A. Cammue P.H. Goodwin S. KrishnaRaj P.K. Saxena (1999) ArticleTitleResistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1 Plant Cell Rep. 18 IssueID10 835–840 Occurrence Handle10.1007/s002990050670 Occurrence Handle1:CAS:528:DyaK1MXktVWqt7g%3D

    Article  CAS  Google Scholar 

  • J.P. Blein P. Coutos-Thevenot D. Marion M. Ponchet (2002) ArticleTitleFrom elicitins to lipid-transfer proteins: a new insight in cell signaling involved in plant defense mechanisms Trends Plant Sci. 7 293–296 Occurrence Handle10.1016/S1360-1385(02)02284-7 Occurrence Handle1:CAS:528:DC%2BD38Xlt1OhtLc%3D Occurrence Handle12119165

    Article  CAS  PubMed  Google Scholar 

  • J.M. Bonman D.J. Mackill (1988) ArticleTitleDurable resistance to rice blast disease Oryza 25 103–110

    Google Scholar 

  • L.A. Bravo T.J. Close L.J. Corcuera C.L. Guy (1999) ArticleTitleCharacterisation of an 80-kDa dehydrin-like protein in barley responsive to cold acclimation Physiol. Plant. 106 177–183 Occurrence Handle10.1034/j.1399-3054.1999.106205.x Occurrence Handle1:CAS:528:DyaK1MXltVersLY%3D

    Article  CAS  Google Scholar 

  • W.F. Broekaert B.P.A. Cammue M.F.C. Bolle ParticleDe K. Thevissen G.W. Samblanx ParticleDe R.W. Osborn (1997) ArticleTitleAntimicrobial peptides in plants Crit. Rev. Plant Sci. 16 297–323 Occurrence Handle1:CAS:528:DyaK2sXktlWntro%3D

    CAS  Google Scholar 

  • N. Buhot J.P. Douliez A. Jacquemard D. Marion V. Tran B.F. Maume M.L. Milat M. Ponchet V. Mike J.C. Kader J.P. Blein (2001) ArticleTitleA lipid transfer protein binds to a receptor involved in the control of plant defense responses FEBS Lett. 509 27–30 Occurrence Handle10.1016/S0014-5793(01)03116-7 Occurrence Handle1:CAS:528:DC%2BD3MXovVCisb4%3D Occurrence Handle11734200

    Article  CAS  PubMed  Google Scholar 

  • B.P. Cammue K. Thevissen M. Hendriks K. Eggermont I.J. Goderis P. Proost J. Damme ParticleVan R.W. Osborn F. Guerbette J.C. Kader (1995) ArticleTitleA potent antimicrobial protein from onion seeds showing sequence homology to plant lipid transfer proteins Plant Physiol. 109 IssueID2 445–55 Occurrence Handle10.1104/pp.109.2.445 Occurrence Handle1:CAS:528:DyaK2MXoslOqsbg%3D Occurrence Handle7480341

    Article  CAS  PubMed  Google Scholar 

  • M.J. Carmona A. Molina J.A. Fernández J.J. López-Fando F. García-Olmedo (1993) ArticleTitleExpression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens Plant J. 3 457–462 Occurrence Handle10.1046/j.1365-313X.1993.t01-24-00999.x Occurrence Handle1:CAS:528:DyaK3sXks1ygsrk%3D Occurrence Handle8220454

    Article  CAS  PubMed  Google Scholar 

  • D.C. Chen B.C. Yang T.T. Kuo (1992) ArticleTitleOne-step transformation of yeast in stationary phase Curr. Genet. 21 83–84 Occurrence Handle10.1007/BF00318659 Occurrence Handle1:CAS:528:DyaK38XhtVams74%3D Occurrence Handle1735128

    Article  CAS  PubMed  Google Scholar 

  • L. Chen S. Zhang R.N. Beachy C.M. Fauquet (1998) ArticleTitleA protocol for consistentlarge scale production of fertile transgenic rice plants Plant Cell Rep. 18 25–31

    Google Scholar 

  • A.H. Christensen P. Quail (1996) ArticleTitleUbiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants Transgenic Res. 5 213–218 Occurrence Handle10.1007/BF01969712 Occurrence Handle1:CAS:528:DyaK28XjsFamsbo%3D Occurrence Handle8673150

    Article  CAS  PubMed  Google Scholar 

  • M.F.C. Bolle ParticleDe R.W. Osborn I.J. Goderis L. Noe D. Acland C.A. Hart S. Torrekens F.V. Leuven W.F. Broekaert (1996) ArticleTitleAntimicrobial peptides from Mirabilis jalapa Amaranthus caudatus: expression, processing, localization and biological activity in transgenic tobacco Plant Mol. Biol. 31 993–1008 Occurrence Handle10.1007/BF00040718 Occurrence Handle8843942

    Article  PubMed  Google Scholar 

  • S.L. Dellaporta J. Wood J.B. Hicks (1983) ArticleTitleA plant DNA minipreparation: version 2 Plant Mol. Biol. Rep. 1 19–22 Occurrence Handle1:CAS:528:DyaL2cXksFWhtrk%3D

    CAS  Google Scholar 

  • W. Dewitte H. Onckelen ParticleVan (2001) ArticleTitleProbing the distribution of plant hormones by immunocytochemistry Plant Growth Regul. 33 67–74 Occurrence Handle10.1023/A:1010729703354 Occurrence Handle1:CAS:528:DC%2BD3MXjtVahtbk%3D

    Article  CAS  Google Scholar 

  • I.E.J.A. Francois M.F.C. Bolle ParticleDe G. Dwyer I.J.W.M. Goderis P.F.J. Woutors P.D. Verhaert P. Proost W.M.M. Schaaper B.P.A. Cammue W.F. Broekaert (2002) ArticleTitleTransgenic expression in Arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins Plant Physiol. 128 1346–1358 Occurrence Handle10.1104/pp.010794 Occurrence Handle1:CAS:528:DC%2BD38XivFylu7w%3D Occurrence Handle11950983

    Article  CAS  PubMed  Google Scholar 

  • A.G. Gao S.M. Hakimi C.A. Mittanck Y. Wu B.M. Woerner D.M. Stark D.M. Shah J. Liang C.M.T. Rommens (2000) ArticleTitleFungal pathogen protection in potato by expression of a plant defensin peptide Nat. Biotechnol. 18 1307–1310 Occurrence Handle1:CAS:528:DC%2BD3cXovVOmsrk%3D Occurrence Handle11101813

    CAS  PubMed  Google Scholar 

  • F. Garcia-Olmedo F. Molina A. Segura M. Moreno (1995) ArticleTitleThe defensive role of non-specific lipid transfer proteins in plants Trends Microbiol. 3 72–74 Occurrence Handle1:STN:280:ByqB2crmtVE%3D Occurrence Handle7728389

    CAS  PubMed  Google Scholar 

  • Y. Hiei S. Ohta T. Komari T. Kumashiro (1994) ArticleTitleEfficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA Plant J. 6 IssueID2 271–282 Occurrence Handle10.1046/j.1365-313X.1994.6020271.x Occurrence Handle1:CAS:528:DyaK2MXhtlGntLc%3D Occurrence Handle7920717

    Article  CAS  PubMed  Google Scholar 

  • F. Hoh J.L. Pons M.F. Gautier F. de Lamotte C. Dumas (2005) ArticleTitleStructure of a liganded type 2 non-specific lipid-transfer protein from wheat and the molecular basis of lipid binding Acta Cryst. D61 397–406 Occurrence Handle1:CAS:528:DC%2BD2MXivVeisr8%3D

    CAS  Google Scholar 

  • T. Iwai H. Kaku R. Honkura S. Nakamura H. Ochiai T. Sasaki Y. Ohashi (2002) ArticleTitleEnhanced resistance to seed transmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall-bound thionin Mol. Plant Microbe Interact. 15 515–521 Occurrence Handle1:CAS:528:DC%2BD38XktlSntrw%3D Occurrence Handle12059099

    CAS  PubMed  Google Scholar 

  • G. Jach B. Gornhardt J. Mundy J. Logemann E. Pinsdorf R. Leah J. Schell C. Maas (1995) ArticleTitleEnhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco Plant J. 8 IssueID1 97–109 Occurrence Handle10.1046/j.1365-313X.1995.08010097.x Occurrence Handle1:CAS:528:DyaK2MXnslSltL8%3D Occurrence Handle7655510

    Article  CAS  PubMed  Google Scholar 

  • J.C. Kader (1996) ArticleTitleLipid-transfer protein in plants Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 627–654 Occurrence Handle10.1146/annurev.arplant.47.1.627 Occurrence Handle1:CAS:528:DyaK28XjtlWgt7w%3D Occurrence Handle15012303

    Article  CAS  PubMed  Google Scholar 

  • H.E. Kauffman A.P.K. Reddy S.P.V. Hsieh S.D. Marca (1973) ArticleTitleAn improved technique for evaluation of resistance of rice varities to Xanthomonas oryzae Plant Dis. Rep. 57 537–541

    Google Scholar 

  • A. Kornberg B.L. Horecker N.O. Kaplan (1955) Meth. Enzym Academic Press New York 323

    Google Scholar 

  • X. Li K. Gasic B.P.A. Cammue W.F. Broekaert S.S. Korban (2003) ArticleTitleTransgenic rose lines harboring an antimicrobial protein geneAce-AMP1demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa) Planta 218 IssueID2 226–232 Occurrence Handle10.1007/s00425-003-1093-5 Occurrence Handle1:CAS:528:DC%2BD3sXptleluro%3D Occurrence Handle14508687

    Article  CAS  PubMed  Google Scholar 

  • A.M. Maldonado P. Doerner R.A. Dixon C.J. Lamb R.K. Cameron (2002) ArticleTitleA putative lipid transfer protein involved in systemic resistance signaling in Arabidopsis Nature 419 399–403 Occurrence Handle10.1038/nature00962 Occurrence Handle1:CAS:528:DC%2BD38XntlGgsLk%3D Occurrence Handle12353036

    Article  CAS  PubMed  Google Scholar 

  • D.A. Mitchell T.K. Marshall R.J. Deschenes (1993) ArticleTitleVectors for the inducible expression of glutathione S-transferase fusion proteins in yeast Yeast. 9 715–723 Occurrence Handle10.1002/yea.320090705 Occurrence Handle1:CAS:528:DyaK3sXlvFKrt7g%3D Occurrence Handle8368005

    Article  CAS  PubMed  Google Scholar 

  • F. Mourgues M.N. Brisset E. Chevreau (1998) ArticleTitleStrategies to improve plant resistance to bacterial diseases through genetic engineering Trends Biotechnol. 16 203–210 Occurrence Handle10.1016/S0167-7799(98)01189-5 Occurrence Handle1:CAS:528:DyaK1cXjt1CgsLc%3D Occurrence Handle9621459

    Article  CAS  PubMed  Google Scholar 

  • A. Pastor M. Lopez-Carbonell L. Alegre (1999) ArticleTitleAbscisic acid immunolocalisation and ultrastructural changes in water-stressed lavender (Lavendula stoechas L.) plants Physiol. Plant. 105 272–279 Occurrence Handle10.1034/j.1399-3054.1999.105212.x Occurrence Handle1:CAS:528:DyaK1MXis1GgtLc%3D

    Article  CAS  Google Scholar 

  • A. Perez-Garcia S. Pereira A. Garcia Gutierrez F. Cazorla R. Salema A. Vicente Particlede F. Canovas (1998) ArticleTitleCytosolic localization in tomato mesophyll cells of a novel glutamine synthetase induced in response to bacterial infection or phosphinothricin treatment Planta 206 426–434 Occurrence Handle1:CAS:528:DyaK1cXlvVeju7c%3D

    CAS  Google Scholar 

  • G.P. Singh M.K. Srivastava R.V. Singh R.M. Singh (1977) ArticleTitleVariation in quantitative and qualitative losses caused by bacterial blight in different rice varieties Indian Phytopathol. 30 180–185

    Google Scholar 

  • G. Sridevi M. Dhandapani K. Veluthambi (2005) ArticleTitle Agrobacterium-mediated transformation of White Ponnia non-basmati variety of indica rice (Oryza sativa L.) Curr. Sci. 88 128–132 Occurrence Handle1:CAS:528:DC%2BD2MXht12rsrs%3D

    CAS  Google Scholar 

  • S. Tassin W.F. Broeckaert D. Marion D.P. Acland M. Ptak F. Vovelle P. Sodano (1998) ArticleTitleSolution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins Biochemistry. 37 3623–3637 Occurrence Handle10.1021/bi9723515 Occurrence Handle1:CAS:528:DyaK1cXht12lsro%3D Occurrence Handle9521681

    Article  CAS  PubMed  Google Scholar 

  • Q. Zhu T. Dabi A. Beeche R. Yamamoto M.A. Lawton C. Lamb (1995) ArticleTitleCloning and properties of a rice gene encoding phenylalanine ammonia lyase Plant Mol. Biol. 29 535–550 Occurrence Handle10.1007/BF00020983 Occurrence Handle1:CAS:528:DyaK28Xit1ejsg%3D%3D Occurrence Handle8534851

    Article  CAS  PubMed  Google Scholar 

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  1. Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, Vadodara, 390002, Gujarat, India

    Rajesh Narhari Patkar & Bharat Bhushan Chattoo

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  1. Rajesh Narhari Patkar
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  2. Bharat Bhushan Chattoo
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Correspondence to Bharat Bhushan Chattoo.

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Patkar, R.N., Chattoo, B.B. Transgenic indica Rice Expressing ns-LTP-Like Protein Shows Enhanced Resistance to Both Fungal and Bacterial Pathogens. Mol Breeding 17, 159–171 (2006). https://doi.org/10.1007/s11032-005-4736-3

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