Abstract
This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of “escapes” during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic potentiality of calli (by a factor of 3.2) and accelerate the transgenic shoot regeneration (by a factor of about 2). On comparison of the transformation rate observed while using low light (LL) and high light (HL) a considerable enhancement from 0.12 to 5.7% was evident. The promotive effect of light might also had a direct beneficial effect on transgenic plant production time leading to a reduction of more than 4 months in the time need to obtain transgenic seeds. All data indicate that HL plays a role on growth and on protein, rubisco and pigment contents by stimulating the gene implicated in photosynthetic and Calvin cycle processes.
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Abbreviations
- BAP:
-
Benzylaminopurine
- Cv:
-
Cultivar
- DAF:
-
Day after flowering
- MES:
-
Morpholino ethane sulfonic acid
- NAA:
-
Naphthalene acetic acid
- PRK:
-
Phosphoribulokinase
- rbs :
-
Gene encoding Rubisco
- rbs Act:
-
Gene encoding Rubisco activase
- GAPDH:
-
Glyceraldehyde 3-phosphate dehydrogenase
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Acknowledgments
The authors would like to thank Biogemma (France) for providing the p35S-gus binary vector, G. Neutelings and S. Hawkins for flax EST information, V. Devillers for technical assistance with flax transformation, and S. Hawkins for helping with English. This work was financially supported by the “Conseil Régional de Picardie” (France).
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Communicated by L. Jouanin.
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Caillot, S., Rosiau, E., Laplace, C. et al. Influence of light intensity and selection scheme on regeneration time of transgenic flax plants. Plant Cell Rep 28, 359–371 (2009). https://doi.org/10.1007/s00299-008-0638-2
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DOI: https://doi.org/10.1007/s00299-008-0638-2