Summary
Using an Agrobacterium tumefaciens binary vector (pAL4404, pBI131), we have demonstrated the transfer of the β-glucuronidase gene into the flax (Linum usitatissimum L.) cultivar Glenelg after selection for kanamycin resistance. The transformed lines were obtained by inoculation and subsequent regeneration of hypocotyl segments. The callus that formed on the cut surfaces of the hypocotyl segments was isolated three weeks after infection and was subsequently subcultured to yield shoots. This procedure generated a large number of transgenic shoots over a relatively short period of time. The transformation efficiencies obtained were the highest reported so far for this plant species.
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Abbreviations
- 2,4-D, 2,4:
-
dichlorophenoxyacetic acid
- GUS:
-
β glucuronidase
- MS:
-
Murasbige and Skoog (1962) medium
- MU:
-
4-methyl-umbelliferone
- MUG:
-
4-methylumbelliferyl-glucuronide
- NPTII:
-
neomycin phosphotransferase II
- PCR:
-
polymerase chain reaction
References
Basiran N, Armitage P, Scott RJ, Draper J (1987) Plant Cell Rep 6: 396–399
Bevan MW (1984) Nucl Acids Res 12: 8711–8721
Bradford MM (1976) Anal Biochem 72: 248–254
Dong JZ, McHughen A (1993) Plant Sci 88: 61–71
Draper ID, Scott RS, Armitage P (1988) Plant Genetic Transformation and Gene expression, a Laboratory Manual. Blackwell Scientific Publications
Edwards K, Johnstone C, Thompson C (1991) Nucl Acids Res 19: 1349
Gamborg OL, Shyluk JP (1976) Bot Gaz 137: 310–306
Hepburn AG, Clarke LE, Blundy KS, White J (1983) Molec Appl Genet 2:211–224
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) Nature 303: 179–180
Jefferson RA (1987) Plant Mol Biol Rep 5: 387–405
Jefferson RA, Kavanagh IA, Bevan MW (1987) EMBO J 6: 3901–3907
Jordan MC, McHughen A (1988a) Plant Cell Rep 7: 281–284
Jordan MC, McHughen A (1988b) Plant Cell Rep 7: 285–287
McHughen A, Browne R, Kneeshaw D, Jordan MC (1986) VI Int Congr IAPIC, Minneapolis USA: 130
McHughen A, Jordan MC, Feist G (1989) J Plant Physiol 135: 245–248
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nap JP, Keizer P, Jansen R (1993) Plant Mol Biol Rep 11: 156–164
Preťová A, Williams EG (1986) J Plant Physiol 126: 155–161
Peach S, Veiten J (1991) Plant Mol Biol 17: 49–60
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, a Laboratory Manual, 2nd Ed. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press
Wilmink A, Dons JJM (1993) Plant Mol Biol Rep 11: 165–185
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Communicated by H. Lörz
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Mlynárová, Ľ., Bauer, M., Nap, JP. et al. High efficiency Agrobacterium-mediated gene transfer to flax. Plant Cell Reports 13, 282–285 (1994). https://doi.org/10.1007/BF00233320
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DOI: https://doi.org/10.1007/BF00233320