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Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana

  • Cell Biology and Morphogenesis
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Abstract

We have investigated the role of ethylene in shoot regeneration from cotyledon explants of Arabidopsis thaliana. We examined the ethylene sensitivity of five ecotypes representing both poor and prolific shoot regenerators and identified Dijon-G, a poor regenerator, as an ecotype with dramatically enhanced ethylene sensitivity. However, inhibiting ethylene action with silver nitrate generally reduced shoot organogenesis in ecotypes capable of regeneration. In ecotype Col-0, we found that ethylene-insensitive mutants (etr1-1, ein2-1, ein4, ein7) exhibited reduced shoot regeneration rates, whereas constitutive ethylene response mutants (ctr1-1, ctr1-12) increased the proportion of explants producing shoots. Our experiments with ethylene over-production mutants (eto1, eto2 and eto3) indicate that the ethylene biosynthesis inhibitor gene, ETO1, can act as an inhibitor of shoot regeneration. Pharmacological elevation of ethylene levels was also found to significantly increase the proportion of explants regenerating shoots. We determined that the hookless1 (hls1-1) mutant, a suppressor of the ethylene response phenotypes of ctr1 and eto1 mutants, is capable of dramatically enhancing shoot organogenesis. The effects of ACC and loss of HLS1 function on shoot organogenesis were found to be largely additive.

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Acknowledgments

This work was supported by an Ontario Premier’s Research Excellence Award (to MNR) and grants from the Ontario Ministry of Agriculture and the Natural Sciences and Engineering Research Council of Canada. Mutant seed stocks were supplied by the Arabidopsis Biological Resource Centre, OH (US). We thank Michael Pautler for his critical reading of this manuscript.

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  1. Department of Plant Agriculture, University of Guelph, 50 Stone Road, Guelph, ON, Canada, N1G 2W1

    Steven P. Chatfield & Manish N. Raizada

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  1. Steven P. Chatfield
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  2. Manish N. Raizada
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Correspondence to Manish N. Raizada.

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Communicated by M. Jordan.

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Chatfield, S.P., Raizada, M.N. Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana . Plant Cell Rep 27, 655–666 (2008). https://doi.org/10.1007/s00299-007-0496-3

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