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Transgenic rice plants expressing trichothecene 3-O-acetyltransferase show resistance to the Fusarium phytotoxin deoxynivalenol

  • Biotic and Abiotic Stress
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Abstract

Fusarium head blight (FHB) is a devastating disease of small grain cereal crops caused by the necrotrophic pathogen Fusarium graminearum and Fusarium culmorum. These fungi produce the trichothecene mycotoxin deoxynivalenol (DON) and its derivatives, which enhance the disease development during their interactions with host plants. For the self-protection, the trichothecene producer Fusarium species have Tri101 encoding trichothecene 3-O-acetyltransferase. Although transgenic expression of Tri101 significantly reduced inhibitory action of DON on tobacco plants, there are several conflicting observations regarding the phytotoxicity of 3-acetyldeoxynivalenol (3-ADON) to cereal plants; 3-ADON was reported to be highly phytotoxic to wheat at low concentrations. To examine whether cereal plants show sufficient resistance to 3-ADON, we generated transgenic rice plants with stable expression and inheritance of Tri101. While root growth of wild-type rice plants was severely inhibited by DON in the medium, this fungal toxin was not phytotoxic to the transgenic lines that showed trichothecene 3-O-acetylation activity. This is the first report demonstrating the DON acetylase activity and DON-resistant phenotype of cereal plants expressing the fungal gene.

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Acknowledgments

We thank Drs. D. McElroy and R. Wu for the gift of vectors with the rice Act1 promoter (McElroy et al. 1991). This research was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).

Author information

Author notes

  1. Shuichi Ohsato

    Present address: Shibata Distinguished Senior Scientist Laboratory, Discovery Research Institute (DRI), RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

  2. Tetsuko Ochiai-Fukuda

    Present address: Laboratory for Neural Cell Polarity, Brain Science Institute (BSI), RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

  3. Isamu Yamaguchi

    Present address: Presidential Office, Agricultural Chemicals Inspection Station (ACIS), 2-772 Suzuki-cho, Kodaira, Tokyo, 187-0011, Japan

  4. Hiroshi Hamamoto

    Present address: Laboratory of Clinical Plant Science, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan

Authors and Affiliations

  1. Plant & Microbial Metabolic Engineering Research Unit and Laboratory for Remediation Research, Discovery Research Institute (DRI) and Plant Science Center (PSC1), RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Shuichi Ohsato, Tetsuko Ochiai-Fukuda, Naoko Takahashi-Ando, Isamu Yamaguchi & Makoto Kimura

  2. Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan

    Takumi Nishiuchi

  3. Department of Plant Pathology, National Agricultural Research Center (NARC), Kannondai 3-1-1, Tsukuba, Ibaraki, 305-8666, Japan

    Shinzo Koizumi

  4. Laboratory for Adaptation and Resistance, Plant Science Center (PSC1), RIKEN, Suehiro 1-7-22, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Hiroshi Hamamoto

  5. Environmental Molecular Biology Laboratory, Discovery Research Institute (DRI), RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Toshiaki Kudo

Authors
  1. Shuichi Ohsato
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  2. Tetsuko Ochiai-Fukuda
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  3. Takumi Nishiuchi
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  4. Naoko Takahashi-Ando
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  5. Shinzo Koizumi
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  6. Hiroshi Hamamoto
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  7. Toshiaki Kudo
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  8. Isamu Yamaguchi
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  9. Makoto Kimura
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Corresponding author

Correspondence to Makoto Kimura.

Additional information

Communicated by A. Atanassov

S. Ohsato and T. Ochiai-Fukuda should be considered as joint first authors.

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Ohsato, S., Ochiai-Fukuda, T., Nishiuchi, T. et al. Transgenic rice plants expressing trichothecene 3-O-acetyltransferase show resistance to the Fusarium phytotoxin deoxynivalenol. Plant Cell Rep 26, 531–538 (2007). https://doi.org/10.1007/s00299-006-0251-1

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  • DOI: https://doi.org/10.1007/s00299-006-0251-1

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