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Regulation of growth of Lilium plantlets in liquid medium by application of paclobutrazol or ancymidol, for its amenability in a bioreactor system: growth parameters

  • Cell Biology and Morphogenesis
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Abstract

Effect of growth retardants (paclobutrazol or ancymidol) was studied in Lilium plantlets growing in liquid culture. A significant increase in leaf chlorophyll, epicuticular wax, plant dry weight and bulb starch contents were found in plantlets treated with growth retardants. A similar increase in the number of leaves, roots and bulbs was also noted. However, total leaf area and the fresh weight increased only marginally. These features resulted in robust plantlets that showed significantly improved ex vitro survival. Based on these features, a comprehensive index (CI) was calculated as a measure of quality of the plantlets, and it correlated well with their ex vitro survival. Treatment of plantlets with 3.4 μM paclobutrazol was found to be the best and its carry over effects were also minimal.

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Abbreviations

ANC:

Ancymidol

BA:

6-Benzyladenine

CI:

Comprehensive index

GA:

Gibberellic acid

PBZ:

Paclobutrazol

Rt#:

Number of roots

TBV:

Total bulb volume

TLA:

Total leaf area

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Acknowledgements

The award of Senior Research Fellowship to the senior author by CSIR, New Delhi is acknowledged. Thanks are due to Mr. PVM Rao for his kind co-operation in photographic works.

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Authors and Affiliations

  1. Division of Biotechnology, Institute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research), P.O. Box 6, Palampur, Himachal Pradesh, 176061, India

    Rajesh Thakur, Anil Sood, Pramod K. Nagar, Subedar Pandey & P. S. Ahuja

  2. Department of Biotechnology, Panjab University, Chandigarh, India

    R. C. Sobti

Authors
  1. Rajesh Thakur
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  2. Anil Sood
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  3. Pramod K. Nagar
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  4. Subedar Pandey
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  5. R. C. Sobti
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  6. P. S. Ahuja
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Correspondence to Rajesh Thakur.

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Communicated by D. Dudits

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Thakur, R., Sood, A., Nagar, P.K. et al. Regulation of growth of Lilium plantlets in liquid medium by application of paclobutrazol or ancymidol, for its amenability in a bioreactor system: growth parameters. Plant Cell Rep 25, 382–391 (2006). https://doi.org/10.1007/s00299-005-0094-1

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  • DOI: https://doi.org/10.1007/s00299-005-0094-1

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