Abstract
Mammalian ovarian tumor suppressor candidate 2 (OVCA2) gene belongs to the family of serine hydrolase (FSH). This study aimed to elucidate the functional similarities of OVCA2 with its yeast homolog genes (FSH1, FSH2, and FSH3) regarding apoptosis. We found that the expression of OVCA2 in Saccharomyces cerevisiae increased production of reactive oxygen species (ROS), decreased cell growth, disturbed mitochondrial morphology, reduced membrane potential, increased chromatin condensation, and externalization of phosphatidylserine (PS) (annexin V/propidium iodide staining) indicating induced apoptotic cell death in yeast. We also showed that complementation of OVCA2 in fsh3Δ cells reduced cell growth and increased the apoptotic phenotypes. Collectively, our results suggest that complementation of human OVCA2 in fsh3Δ cells induced apoptosis in S. cerevisiae.
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Acknowledgement
The authors would like to acknowledge the infrastructure facilities from DST-FIST, Department of Biochemistry, and DST-PURSE (confocal microscopy) of Bharathidasan University. We also thank Prof. Ram Rajasekharen, CSIR-CFTRI, Mysore, India, and Prof. Benedikt Westermann, Universitat Bayreuth, Germany respectively for providing the deletion strains and pYX223-mtGFP plasmid.
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VN and RG conceived and designed the work and wrote the manuscript; RG, CR conducted the experiments. All authors analyzed the data and reviewed the manuscript.
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Gowsalya, R., Ravi, C. & Nachiappan, V. Human OVCA2 and its homolog FSH3-induced apoptosis in Saccharomyces cerevisiae. Curr Genet 67, 631–640 (2021). https://doi.org/10.1007/s00294-021-01171-6
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DOI: https://doi.org/10.1007/s00294-021-01171-6