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DNA markers define plastid haplotypes in Arabidopsis thaliana

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Abstract

To identify genetic markers in the Arabidopsis thaliana plastid genome (ptDNA), we amplified and sequenced the rpl2-psbA and rbcL-accD regions in 26 ecotypes. The two regions contained eight polymorphic sites including five insertions and/or deletions (indels) involving changes in the length of A or T mononucleotide repeats and three base substitutions. The 27 alleles defined 15 plastid haplotypes, providing a practical set of ptDNA markers for the Columbia, Landsberg erecta and Wassilewskija ecotypes that are commonly used in genetic studies and also for the C24 and RLD ecotypes that are the most amenable for cell culture manipulations.

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Acknowledgments

We thank Peter E. Smouse, Rutgers University, for his guidance on the phylogenetic analyses of the data. This research was supported by the NSF Eukaryotic Genetics Program (MCB—039958) and the USDA Biotechnology Risk Assessment Research Grant Program (2004-39454-15192).

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Authors and Affiliations

  1. Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ, 08854-8020, USA

    Arun K. Azhagiri & Pal Maliga

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  1. Arun K. Azhagiri
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  2. Pal Maliga
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Correspondence to Pal Maliga.

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Communicated by R. Bock.

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Azhagiri, A.K., Maliga, P. DNA markers define plastid haplotypes in Arabidopsis thaliana . Curr Genet 51, 269–275 (2007). https://doi.org/10.1007/s00294-006-0118-6

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  • DOI: https://doi.org/10.1007/s00294-006-0118-6

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