Summary.
An efficient and fast regeneration system from cotyledon protoplasts was established for Arabidopsis thaliana accessions C24, Columbia, and Wassilewskija. Culture conditions and media compositions were optimised for the development of protoplasts embedded in thin alginate layers. Unexpectedly, the absence of cytokinins had a positive effect on cell development. Moreover, combined adjustment of α-naphthylacetic acid and dicamba concentrations resulted in high plating efficiencies of up to 30%, followed by shoot regeneration within only 19 days after protoplast isolation. The protocol is reproducible, efficient, extremely fast, and regenerated plants are fertile. Thus, this cotyledon-based system could prove useful for studying plant cell and molecular biology in A. thaliana.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Additional information
Correspondence and reprints: Zentrum für angewandte Biowissenschaften, Sonnenstrasse 5, 79104 Freiburg, Federal Republic of Germany.
Received December 9, 2002; accepted April 13, 2003; published online September 23, 2003
Rights and permissions
About this article
Cite this article
Dovzhenko, A., Dal Bosco, C., Meurer, J. et al. Efficient regeneration from cotyledon protoplasts in Arabidopsis thaliana . Protoplasma 222, 107–111 (2003). https://doi.org/10.1007/s00709-003-0011-9
Issue Date:
DOI: https://doi.org/10.1007/s00709-003-0011-9