Abstract
The objective of this work is to investigate the hydrolytic degradation of the Monocryl (PGA/PCL) surgical suture in different pH buffer solutions, and its correlation with the structural alterations the material undergoes. To this end, an in vitro degradation study was conducted under acidic (pH = 2), physiological (pH = 7.4), and alkaline (pH = 8.5) conditions at 37 °C, over 25 days. Changes in the swelling rate, structural and mechanical properties of the Monocryl sample with the degradation time were characterized, from which the related degradation mechanism of the material was concluded. Results showed that the structural values of the Monocryl sample were more sensitive in the alkaline medium than the acidic and neutral ones. It exhibited a reduction in birefringence values by 11.5% from the original one in the buffer solution of pH = 8.5, only 4% at pH = 2, and 2.6% at pH = 7.4, after 20 days of degradation durations. Over the same time period, mechanical loss in neutral, acidic, and alkaline media was decreased to 19, 14.9, and 8.3%, respectively. The obtained results revealed that the Monocryl suture exhibits enhanced degradation properties in neutral conditions rather than both acidic and alkaline ones, with a more homogeneous degradation behavior during the degradation process.
Graphical abstract
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Resorbable poly (\(\alpha\)-hydroxy acids), namely polyglycolide (PGA), polylactide (PLA), and their copolymers with epsilon-caprolactone, has been employed in most domains of biomedical applications [1, 2]. These applications include regeneration implants, drug delivery, substrates for tissue engineering, surgical sutures, and others [1,2,3,4,5]. Such materials have the advantages of excellent biocompatibility, low immunogenicity, good handling ability, and biodegradability [2, 6]. Practically, the degradation behavior of these materials is considered one of the crucial steps of tissue regeneration [7]. Due to the fact that the degradation proceeds through hydrolysis of their ester bonds into compounds naturally present in the organism, which are eventually resorbed [8, 9]. Therefore, avoiding the need for a second surgery to remove the implants, which supports cell viability, cell growth, and remodeling of tissues properly [8].
Ideally, biomedical materials used as surgical sutures should have in vivo degradation matches with the new tissue formation [10]. Because if the rate of degradation is too slow, the residue of the surgical suture will hinder cell growth and tissue differentiation. On the other hand, if the degradation rate is too fast, the suture would damage and lose its usefulness before new tissue formation [10]. Therefore, the degradation rate of the implanted material must be consistent with the tissue healing and provide adequate strength until self-function is restored [10, 11]. The degradation behavior is affected by many factors such as chemical composition, chain microstructure, temperature, enzymes, and tissues [12]. Furthermore, the pH level dramatically affects the degradation rate of the implants, particularly if the pH level of tissue or body fluids deviates significantly from the neutral level [2, 13].
The pH is an indispensable indicator for judging homeostasis in the body [14]. In the human body, blood has buffering molecules, such as hemoglobin and albumin, with a pH-scale range between 7.35 and 7.45 [15]. Moreover, the pH of the pancreatic juice in the duodenum ranges from 7.5 to 8.5. Conversely, the major components of gastric juices (gastric acid and digestive enzymes) in the stomach have a pH-scale often ranges between 1.35 and 3.5 [14, 16]. These variations in pH may change the reaction rates of polymer surgical sutures with these different organs of the human body [14]. Consequently, investigating the influence of pH variation on optical, structural, and mechanical properties is a crucial task. Clinically, studying the biomechanical properties of the surgical sutures are crucial for surgeons not only to guarantee a proper load of the used suture but also, it is useful for predicting the retention of the surgical knots [11], since it should be strong enough to resist the postoperative loading conditions in real life [10, 11]. However, the in vivo experiments of such implants are limited by time and experimental costs. Accordingly, the in vitro degradation investigation is essential for providing an initial estimate for the in vivo degradation behavior of the implanted materials [17].
Nowadays, the advances in optical technologies enable us to utilize interferometric techniques for quick and reliable data acquisition on the structure, opto-mechanical, and physical properties of synthetic fibers and sutures [18,19,20,21]. These techniques can be classified into two-beam and multiple-beam interference techniques, which accurately measure significant parameters including refractive index, spectral dispersion, polarizability, birefringence, and their variations with the thermal and mechanical treatments [22, 23]. All of these parameters depend mainly on demodulating the optical phase object that is encoded in the form of two-dimensional (2D) fringe patterns. Accordingly, numerous authors have proposed several algorithms for accurate fringe analysis and obtaining the desired information of tested objects [24, 25]. The spatial carrier frequency technique based on Fourier Transform (FT) algorithm is recognized as the most successful approach in phase retrieval for its high reliability and accuracy [25].
In this paper, we intensively investigate the effects of different pHs on the degradation behavior of the Monocryl (PGA/PCL) surgical suture. To perform this task, an in vitro degradation duration from 1 to 25 days was carried out. We systematically evaluated the structural and optical properties of the suture samples at different degradation times based on the two-beam Mach–Zehnder interferometer attached to the mechanical drawing device. Furthermore, the degradation of the mechanical properties in different simulated fluids in terms of Young’s modulus (Y), Ultimate tensile strength (UTS), and compressibility (\(\chi )\) was calculated.
Material and experimental techniques
Material
The tested suture is Polyglecaprone 25(PGA/PCL) under the trade name (MONOCRYL®). It was fabricated by bulk ring-opening co-polymerization, according to a method described previously [7]. The Monocryl suture is FDA-approved for clinical use, in the form of an absorbable, and monofilament structure with a 75/25 molar ratio (PGA/PCL) with USP size 6/0 [26,27,28,29]. It has unique biocompatibility and absorbability, which make it frequently utilized in most soft tissue applications and smooth muscle tissue engineering [27, 28].
Optical system
For investigating the opto-mechanical properties of the Monocryl suture sample, the two-beam Mach–Zehnder interferometer is constructed as given in Fig. 1a. It is composed of a He–Ne laser source of intensity of 20 mwatt and wavelength of 632.8 nm, a collimating lens, two analogous beam splitters (BS1 and BS2), two identical mirrors (M1 and M2), a spatial filter (SP), polarizer (P), microscope objective lens (MO) with magnification 10x, charged coupled device (CCD Camera), and monitor [25]. This system is provided with a mechanical drawing device to automatically draw the sample at defined draw ratios as shown in Fig. 1b.
In vitro degradation process at different pHs values
Here, samples of Monocryl surgical sutures were individually incubated in 50 ml glass vials. These vials were filled with different-buffer solutions to simulate the acidic, physiological, and alkaline conditions, which were adjusted to (pH = 2.0 \(\pm 0.1)\), (pH = 7.4 \(\pm 0.1)\), and (pH = 8.5 \(\pm 0.1)\), respectively. All samples were kept at a constant temperature of 37 \(^\circ{\rm C}\). It was assured that the fluid completely covered the threads. At various durations of 5, 10, 15, 20, and 25 days, the samples were extracted from the medium and washed with de-ionized water. Then, these samples were blotted dry for 30 s for the following investigations and characterizations. All steps of the in vitro incubation method are illustrated in Fig. 2.
Results and discussions
Investigating the swelling rate in different pH buffer solutions
The degradation mechanism of aliphatic polyesters, like the Monocryl sutures, is based on a hydrolytic cleavage of the ester bonds on their backbone chains. This mechanism is mainly attributed to changes in the water uptake of the degraded samples [29]. So, we focus on investigating the swelling rate of Monocryl sutures in buffer solution with different pH values. The swelling rate percentage (%) for each degradation duration and pH value for the incubated samples were calculated using the following formula [30], and the obtained results are given in Fig. 3:
where \({D}_{a}\) presents the diameter of the Monocryl suture at each degradation duration and pH value, and \({D}_{b}\) refers to the original diameter of the Monocryl suture sample.
From Fig. 3, one can observe that the degree of swelling shows a different tendency at each pH value. Monocryl samples degraded at acidic conditions (pH = 2), and neutral conditions (pH = 7.4) underwent a two-staged change in swelling rate, increasing rapidly to saturation value in the first 15 days and then, decreasing in the rest of the time interval (25 days). In addition, Monocryl samples degraded at pH = 7.4 show a slight decrease in absorption capacity compared to Monocryl samples degraded at pH = 2. For the Monocryl samples degraded at alkaline conditions (pH = 8.5), the water uptake slowly increased with increasing the degradation duration, reaching the maximum value at 20 days. At 25 days’ post-immersion, it was not possible to handle the sample to measure its swelling degree due to its fragile state.
The variations in the degradation behavior for the Monocryl samples at different pH can be correlated with the effect of pH on hydrophilicity. The Monocryl suture is composed of 75:25 PGA/PCL molar ratio formulations. So, the hydrophilic unit of PGA is considered the predominant component in this copolymer structure. Therefore, at a neutral medium (pH = 7.4), the Monocryl sample preserves its hydrophilic nature. Hence, water molecules can enter the copolymer structure via Hydrogen bonds that lead to an increase in the sample diameter. Then, the intrusion of water molecules triggers a hydrolytic attack that cleaves the bonds within chains and eventually erodes the sample. Similarly, at an acidic medium (pH = 2), the hydrophilicity is increased, which promotes further water molecules entering the entire polymer matrix. This explains the observed increase in their absorption capacity. On the other hand, the copolymer in the alkaline medium (pH = 8.5) changed from hydrophilic to hydrophobic (polar) in character during its implantation. Probably, because hydroxyl ions are entrapped by the ester groups on the sample surface, which lowers its absorption capacity. As a result, water molecules slowly penetrate the sample [27, 31].
Investigating the optical properties of Monocryl suture in different pH buffer solutions
The two-beam Mach–Zehnder interferometer was used for investigating the refractive indices and birefringence of Monocryl suture at different pH values during various incubation durations. Each incubated Monocryl suture at a defined pH value and duration was fixed on a glass slide and immersed in a liquid of a suitable refractive index for reducing the refraction inside the suture. Then, they transmitted to the optical setup of Mach–Zehnder interferometer. By adjusting the polarizer, interference patterns for Monocryl suture at each incubation condition were recorded for light waves vibrating parallel (||) and perpendicular (⊥) to the suture axis. For demodulating the phase maps of these interference patterns, the spatial carrier frequency technique was implemented [25]. Figure 4a–c shows the obtained wrapped phase maps of the incubated Monocryl sutures in buffer solution of various pH values at degradation durations of 5, 15, and 25 days, respectively.
After unwrapping phase maps, the refractive indices (n∥ (x, y) & n⊥ (x, y)) for ∥ and ⊥ polarization directions and the birefringence (∆n(x, y)) for each incubated Monocryl suture were calculated using the following relations [32]:
where λ is the wavelength of the used monochromatic light source, \({\varphi }^{\parallel }\left(x,y\right)\; \mathrm{and}\; {\varphi }^{\perp }(x,y)\) are the demodulated unwrapping phase maps at the ∥ and ⊥ polarization directions, respectively, t is the thickness of the sample, and \({n}_{L}\) is the refractive index of immersion liquid. Figure 5a–c illustrates the refractive indices and the birefringence values of the Monocryl sutures as a function of degradation durations at incubated conditions with pH = 2, 7.4, 8.5, respectively.
Figure 5a–c showed a significant decline in refractive indices (∥ and ⊥) and birefringence of the Monocryl sutures with increasing the degradation time. Also, from this figure one can note that the degraded Monocryl samples under alkaline conditions exhibited much more reduction in refractive indices and birefringence values than those obtained from acidic and neutral ones, respectively. For instance, after 20 days of degradation, the birefringence values of Monocryl samples decreased by 11.5% from the original one in the buffer solution of pH = 8.5, only 4% at pH = 2 and 2.6% at pH = 7.4. These results could be attributed to the fact that the surfaces of Monocryl samples are more susceptible to corrosive degradation in an alkalescent environment, which has a negative influence on the degradation rate compared to acidic and neutral environments [10]. Thus, a possible explanation for these results is that the hydrolytic cleavage of the ester groups from the PGA/PCL block in the alkaline medium is fast compared to acidic and neutral ones. Therefore, the loss in the refractive indices and birefringence values of the Monocryl sutures in acidic and neutral media is lower than those of samples degraded in the alkaline medium.
Investigating the opto-mechanical features and durability properties of Monocryl sutures in different pH buffer solutions
Investigating the influence of cold drawing on the structural features of the incubated Monocryl sutures in various pH media is crucial for the successful postoperative period following any suturing surgery. Therefore, the two-beam Mach–Zehnder interferometer equipped with a mechanical cold drawing device (see Fig. 1a, b) was utilized to perform this task for each incubated Monocryl suture at a defined pH value and duration. Here, each incubated Monocryl sample was fitted with the clamps of the mechanical cold drawing device and immersed in a liquid of appropriate refractive index. The mechanical drawing device was fixed into the optical system of the Mach–Zehnder interferometer. Then, the Monocryl suture sample was continuously drawn at defined draw ratios by the stepper motor of the mechanical device. As a result, the material gradually hardens until failure has occurred. To define the value of the draw ratio, the control unit of the mechanical drawing device was utilized. Interference patterns for each incubated Monocryl sample at defined draw ratios were recorded for the ∥ and ⊥ polarization directions.
The phase maps of these recorded interference patterns were demodulated using the spatial carrier frequency technique. Figures 6, 7a–c, and 8a, b present the wrapped phase maps of the drawn Monocryl samples at defined draw ratios and various pH media (2, 7.4, and 8.5) with degradation durations 5, 15, and 25 days, respectively. After unwrapping the obtained wrapped phase maps in Figs. 6, 7a–c, and 8a, b, the refractive indices of the drawn Monocryl sutures are determined. Figures 9 and 10a–c show the refractive indices values of the drawn Monocryl sutures at different draw ratios as a function of degradation durations at incubated conditions pH = 2, 7.4, 8.5 for the ∥ and ⊥ polarization directions, respectively. According to Figs. 9 and 10a–c, at the early stage of incubation (5 and 10 days), all groups showed an increase in parallel and a subsequent decrease in perpendicular refractive indices. With further increases in incubation time, the refractive indices of each group of Monocryl sutures slow down. This confirms the two naturally occurring mechanisms during degradation, recrystallization (formation of new crystalline regions), and fracture of crystallinity (attacking the crystalline phase.)
For studying the durability properties of the Monocryl surgical suture material, the Young’s modulus (\({Y}_{E}\)) and the Ultimate tensile strength (UTS) are considered the most important factors that reflect their hardness and flexibility [33]. For calculating these parameters, the stress–strain curves of the Monocryl suture at each hydrolytic medium are investigated as presented in Fig. 11. From this figure, the Young’s modulus of the incubated Monocryl samples is evaluated by finding the initial linear slope for each curve [9]. Also, the ultimate tensile strength of the incubated Monocryl samples can be evaluated by finding the maximum stress for each stress–strain curve, and their results are tabulated in Table 1. This table clarifies that, there is a superior mechanical strength of the Monocryl suture sample at pH = 7.4 concerning both Young’s modulus and ultimate tensile strength, compared with other media. More analytically, the obtained results indicate that the degraded samples at pH = 8.5 show significantly lower Young’s modulus and ultimate stress than those with pH = 7.4 and 2. For instance, pH 8.5 samples exhibit a 19% decrease in Young’s modulus compared with pH 7.4 (from 270.63 to 205.76 MPa) and 9% compared with pH 2 (from 240.63 to 205.76 MPa) by day 5 of incubation time. Based on the previous results, degradation consists of two steps: Firstly, begins with random breakage of ester linkages leading to a reduction in mechanical properties. Secondly, with a further increase in ester bond breakage, the number of entangled chains decreases resulting in a faster decrease in the mechanical properties of the suture. But, the alkaline environment accelerates this reduction, suggesting that the surface of the PGA/PCL sample rapidly erodes, which catalyzes further degradation of their mechanical properties. The obtained results are in good agreement with the obtained results in Ref [16].
On the basis of Young's modulus values, the compressibility (\(\chi )\) of the Monocryl suture was measured using the following equation [19]:
where μ is the Poisson's ratio (the ratio between the lateral and axial strains). Figure 12a–c illustrates the compressibility (\(\chi )\) for the Monocryl suture as a function of the degradation time at incubated media pH = 2, 7.4, and 8.5, respectively. As expected, there is an increment tendency in compressibility during the entire degradation period. Noticeably, this increment is significant with the high draw ratios. Our findings revealed that the degradation of the mechanical properties is accelerated either by an acidic or basic medium.
Conclusions
In conclusion, a 25-day in vitro degradation experiment is conducted on the Monocryl surgical suture to study the effect of varying pH of the buffer solutions on their hydrolytic degradation. A Mach–Zehnder interferometer attached to a mechanical drawing device is utilized to examine the alterations in structural and opto-mechanical properties of the Monocryl sutures induced by the degradation process. The behavior of the Monocryl suture is varied according to environmental pH. There were considerable differences in the evolution of the degree of swelling with degradation time particularly, in the alkaline medium. Moreover, after 20 days of degradation, the reduction in the birefringence values of Monocryl samples in the buffer solution of pH = 8.5 is being more advanced. Noticeably, mechanical loss values of the Monocryl suture in neutral, acidic, and alkaline media after degradation of 5 days were decreased to 78, 69, and 58%, respectively, suggesting that Monocryl suture in both acidic and alkaline environments showed a faster degradation rate in their mechanical properties than those in the neutral one. Based on the comparison of the three pH values, the neutral condition (pH = 7.4) is preferable for the Monocryl suture implantation. The obtained results may provide preliminary experimental references for evaluating the degradation performance of the Monocryl surgical suture.
Availability data and material
Datasets used and analyzed during the current study are available from the author on reasonable request.
References
Prasad A (2021) Bioabsorbable polymeric materials for biofilms and other biomedical applications: recent and future trends. Mater Today Proc 44:2447–2453. https://doi.org/10.1016/j.matpr.2020.12.489
Josyula A, Parikh KS, Pitha I, Ensign LM (2021) Engineering biomaterials to prevent post-operative infection and fibrosis. Drug Deliv Transl Res 11:1675–1688. https://doi.org/10.1007/s13346-021-00955-0
Nair LS, Laurencin CT (2007) Biodegradable polymers as biomaterials. Prog Polym Sci 32:762–798. https://doi.org/10.1016/j.progpolymsci.2007.05.017
Nazir F, Abbas L, Iqbal M (2022) A comparative insight into the mechanical properties, antibacterial potential, and cytotoxicity profile of nano-hydroxyapatite and nano-whitlockite-incorporated poly-L-lactic acid for bone tissue engineering. Appl Nanosci 12:47–68. https://doi.org/10.1007/s13204-021-02223-6
Sun J, Walker J, Beck-Broichsitter M, Schwendeman SP (2022) Characterization of commercial PLGAs by NMR spectroscopy. Drug Deliv Transl Res 12:720–729. https://doi.org/10.1007/s13346-021-01023-3
Johari N, Fathi MH, Golozar MA et al (2012) Poly(e-caprolactone)/nano fluoridated hydroxyapatite scaffolds for bone tissue engineering: In vitro degradation and biocompatibility study. J Mater Sci Mater Med 23:763–770. https://doi.org/10.1007/s10856-011-4528-8
Kasperczyk J, Li S, Jaworska J et al (2008) Degradation of copolymers obtained by ring-opening polymerization of glycolide and ε-caprolactone: a high resolution NMR and ESI-MS study. Polym Degrad Stab 93:990–999. https://doi.org/10.1016/j.polymdegradstab.2008.01.019
Wen W, Zou Z, Luo B, Zhou C (2017) In vitro degradation and cytocompatibility of g-MgO whiskers/PLLA composites. J Mater Sci 52:2329–2344. https://doi.org/10.1007/s10853-016-0525-0
Hamza AA, El-Bakary MA, Ibrahim MA, Elgamal MA, El-Sayed NM (2022) Investigate the degradable behavior of a poly (glycolide-co-trimethylene carbonate) suture material used in a vascular surgery. Polym Bull 79(12):10783–10801. https://doi.org/10.1007/s00289-021-04070-5
Karabulut R, Sonmez K, Turkyilmaz Z et al (2010) An in vitro and in vivo evaluation of tensile strength and durability of seven suture materials in various pH and different conditions: an experimental study in rats. Indian J Surg 72:386–390. https://doi.org/10.1007/s12262-010-0158-5
Kim JC, Lee YK, Lim BS et al (2007) Comparison of tensile and knot security properties of surgical sutures. J Mater Sci Mater Med 18:2363–2369. https://doi.org/10.1007/s10856-007-3114-6
Niculescu M, Antoniac A, Vasile E et al (2016) Evaluation of biodegradability of surgical synthetic absorbable suture materials: An in vitro study. Mater Plast 53:642–645
Dragovic M, Pejovic M, Stepic J et al (2020) Comparison of four different suture materials in respect to oral wound healing, microbial colonization, tissue reaction and clinical features—randomized clinical study. Clin Oral Investig 24:1527–1541. https://doi.org/10.1007/s00784-019-03034-4
Freudenberg S, Rewerk S, Kaess M et al (2004) Biodegradation of absorbable sutures in body fluids and pH buffers. Eur Surg Res 36:376–385. https://doi.org/10.1159/000081648
Mieno H, Marunaka Y, Inaba T et al (2019) pH balance and lactic acid increase in the vitreous body of diabetes mellitus patients. Exp Eye Res 188:107789. https://doi.org/10.1016/j.exer.2019.107789
Tomihata K, Suzuki M, Ikada Y (2001) The pH dependence of monofilament sutures on hydrolytic degradation. J Biomed Mater Res 58:511–518. https://doi.org/10.1002/jbm.1048
Li YL, He J, Ye HX et al (2022) Atomic layer deposition of zinc oxide onto 3D porous iron scaffolds for bone repair: in vitro degradation, antibacterial activity and cytocompatibility evaluation. Rare Met 41:546–558. https://doi.org/10.1007/s12598-021-01852-8
Sokkar TZN, El FKA, Bakary MA, Omar EZ, Agour M (2017) Characterization of axially tilted fibres utilizing a single-shot interference pattern. Opt Lasers Eng 91:144–150. https://doi.org/10.1016/j.optlaseng.2016.11.018
El-Sayed NM, El-Bakary MA, Ibrahim MA, Elgamal MA, Hamza AA (2021) Characterization of the mechanical and structural properties of PGA/TMC copolymer for cardiac tissue engineering. Microsc Res Tech 84:1596–1606. https://doi.org/10.1002/jemt.23720
El-Bakary MA (2008) Determining the opto-mechanical and geometrical properties of high-density polyethylene fibres. Opt Lasers Eng 46:328–335. https://doi.org/10.1016/j.optlaseng.2007.11.007
El-Rashidy NM, El-Bakary MA, Omar EZ, El-Sayed NM, Hamza AA (2022) Phase estimation for investigating the optical and mechanical properties of Monocryl suture for soft tissue approximation and ligation. Microsc Res Tech 85(10):3455–3465. https://doi.org/10.1002/jemt.24201
Hamza AA, Fouda IM, Sokkar TZN, El-Bakary MA (1999) Determining the spectral dispersion curves of polypropylene fibres. J Opt A Pure Appl Opt 1:359–366. https://doi.org/10.1002/app.10602
Hamza AA, Sokkar TZN, El-Bakary MA, Ali AM (2003) An interferometric method for studying the influence of temperature on the mean refractive indices and cross- sectional area of irregular fibres. Polym. Test 22:83–91. https://doi.org/10.1016/S0142-9418(02)00053-3
Omar EZ (2020) A computational phase-shifting interferometric method using a single-shot microinterferogram and its application Optik (Stuttg) 217:164924 https://doi.org/10.1016/j.ijleo.2020.164924.
Sokkar TZN, El-Farahaty KA, El-Bakary MA, Omer EZ, Hamza AA (2016) A modified method for accurate correlation between the craze density and the optomechanical properties of fibers using pluta microscope. Microsc Res Tech 79(5):422–430. https://doi.org/10.1002/jemt.22645
Runk A, Allen SW, Mahaffey EA (1999) Tissue reactivity to poliglecaprone 25 in the feline linea alba. Vet Surg 28:466–471. https://doi.org/10.1111/j.1532-950X.1999.00466.x
Junge K, Rosch R, Krones CJ et al (2005) Influence of polyglecaprone 25 (Monocryl) supplementation on the biocompatibility of a polypropylene mesh for hernia repair. Hernia 9:212–217. https://doi.org/10.1007/s10029-004-0315-5
Molea G, Schonauer F, Bifulco G, D’Angelo D (2000) Comparative study on biocompatibility and absorption times of three absorbable monofilament suture materials (Polydioxanone, Poliglecaprone 25, Glycomer 631). Br J Plast Surg 53:137–141. https://doi.org/10.1054/bjps.1999.3247
Vieira AC, Vieira JC, Guedes RM, Marques AT (2010) Degradation and viscoelastic properties of PLA-PCL, PGA-PCL, PDO and PGA fibres. Mater Sci Forum 636–637:825–832. https://doi.org/10.4028/www.scientific.net/MSF.636-637.825
Zhang Z, Han X, Du W et al (2019) Comparison and evaluation of in vitro degradation behaviors of organosilicone-modified gelatin hybrids. J Sol-Gel Sci Technol 89:370–379. https://doi.org/10.1007/s10971-018-4883-8
Sailema-Palate GP, Vidaurre A, Campillo AF, Castilla-Cortázar I (2016) A comparative study on Poly(ε-caprolactone) film degradation at extreme pH values. Polym Degrad Stab 130:118–125. https://doi.org/10.1016/j.polymdegradstab.2016.06.005
Barakat N, Hamza AA (1990) Interferometry of fibrous materials. Adam Hilger, Bristol
Ferrara M, Lugano G, Sandinha MT et al (2021) Biomechanical properties of retina and choroid: a comprehensive review of techniques and translational relevance. Eye 35:1818–1832. https://doi.org/10.1038/s41433-021-01437-w
Funding
Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB). It is not applicable for this study.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The author declare that they have no competing interests.
Ethical approval
It is not applicable for this study.
Consent for publication
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Hamza, A.A., El-Bakary, M.A., El-Rashidy, N.M. et al. The influence of degradation in different pH buffer solutions on the optical and durability properties of Monocryl suture: (an in vitro study). Polym. Bull. 81, 3149–3168 (2024). https://doi.org/10.1007/s00289-023-04843-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00289-023-04843-0