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Facile synthesis of boronic acid-functionalized nanocarriers for glucose-triggered caffeic acid release

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Abstract

In this study, boronic acid-functionalized methacrylate-based nanoparticles were synthesized via surfactant-free emulsion polymerization in one pot. Uniform (polydispersity index <0.05) sub-100 nm nanoparticles were obtained. The changes of average hydrodynamic diameter and polydispersity index of nanoparticles against boronic acid content in total monomer and acetone percentage in the solvent mixture were investigated. Polymerization kinetics in terms of monomer conversion rate was monitored by gravimetric method. The nanoparticles were characterized by scanning electron microscopy and dynamic light scattering. The boron content in the nanoparticles was confirmed by electron-dispersive X-ray spectroscopy. Further, the nanoparticles were combined with caffeic acid. Caffeic acid carrying nanoparticles were titrated against glucose or fructose in which caffeic acid is released by the displacement reaction in a controlled manner. The displacement of caffeic acid and glucose was monitored by UV–visible spectral change. Furthermore, in vitro biocompatibility of nanoparticles was tested in NIH-3T3 cells, which resulted no significant toxicity effect on the cells.

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Acknowledgments

This work was supported by Selcuk University Scientific Research Fund (S.U. BAP No. 12201077) and TUBITAK (Project No.112M096, COST TD1004). The authors are grateful to Advanced Technology Research and Application Center of Selcuk University for their kind help regarding with analysis studies (SEM, EDX, DLS, UV–Vis). The authors thank to Dr. Pembegul UYAR for providing 3T3 cells as a kind gift and her valuable guidance and to Huseyin Sakalak for his help in experimental studies.

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Authors and Affiliations

  1. Higher School of Health Services, Mevlana University, Yeni Istanbul Cad. No. 235, Selcuklu, 42003, Konya, Turkey

    Mehmet Ulasan

  2. Advanced Technology Research and Application Center, Selcuk University, Selcuk University Campus, Selcuklu, 42070, Konya, Turkey

    Mehmet Ulasan, Emine Yavuz & Mustafa Selman Yavuz

  3. Department of Immunology, Gazi University, 06560, Ankara, Turkey

    Emine Yavuz

  4. Department of Chemistry, Selcuk University, 42070, Konya, Turkey

    Yunus Cengeloglu

  5. Metallurgy and Materials Engineering, Selcuk University, 42070, Konya, Turkey

    Mustafa Selman Yavuz

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  1. Mehmet Ulasan
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Correspondence to Mehmet Ulasan or Mustafa Selman Yavuz.

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Ulasan, M., Yavuz, E., Cengeloglu, Y. et al. Facile synthesis of boronic acid-functionalized nanocarriers for glucose-triggered caffeic acid release. Polym. Bull. 72, 2127–2142 (2015). https://doi.org/10.1007/s00289-015-1393-5

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  • DOI: https://doi.org/10.1007/s00289-015-1393-5

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