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Description of Mycolicibacterium arenosum sp. nov. Isolated from Coastal Sand on the Yellow Sea Coast

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Abstract

A Gram-staining-positive, aerobic, non-spore-forming bacterium was isolated from coastal sand samples from Incheon in the Republic of Korea and designated as strain CAU 1645T. The optimum conditions for growth were observed at 30 °C in growth media containing 1% (w/v) NaCl at pH 9.0. The predominant respiratory quinone was MK-9 and the major fatty acids were C16:0, C17:1 w7c, and summed feature 7. Similarly, the 16S rRNA gene sequence exhibited the highest similarity with Mycolicibacterium bacteremicum DSM 45578T and Mycolicibacterium neoaurum JCM 6365T, both of which exhibited similarity rates of 97.2%. The genomic DNA G+C content was 68.2%. The whole genome of strain CAU 1645T was obtained and annotated with annotation using RAST server. The pan-genome analysis was determined using Prokka, Roary, and Phandango. In the pan-genome analysis, the strain CAU 1645T shared 40 core genes with closely related Mycolicibacterium species, including the AcpM gene, the meromycolate extension acyl carrier protein involved in forming impermeable cell walls in mycobacteria. Therefore, our findings demonstrated that the isolate represents a novel species of the genus Mycolicibacterium, for which we propose the name Mycolicibacterium arenosum sp. nov. The type strain is CAU 1645T (= KCTC 49724T = MCCC 1K07087T).

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Data Availability

The GenBank/EMBL/DDBJ accession numbers of strain CAU 1645T including 16S rRNA gene sequence are OK053000 and the genomic sequence is JANDBD000000000.

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Funding

This work was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of the Environment (MOE) of the Republic of Korea (NIBR202102205), the National Research Foundation of Korea (NRF) (NRF-2021R1C1C2003223), and Chung-Ang University Young Scientist Scholarship in 2017.

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Author notes

  1. Jiseon Jeong, Soyeon Ahn, Thoi Cong Truong, and Jong-Hwa Kim have contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea

    Jiseon Jeong, Soyeon Ahn, Thoi Cong Truong, Jong-Hwa Kim, Veeraya Weerawongwiwat & Wonyong Kim

  2. Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea

    Jung-Sook Lee

  3. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea

    Jung-Hoon Yoon

  4. Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkla, 90110, Thailand

    Ampaitip Sukhoom

Authors
  1. Jiseon Jeong
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  2. Soyeon Ahn
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Contributions

WK conceived and designed the study. JJ, SA, TCT, VW, and J-SL performed the experiments. J-HK, J-HY, and AS carried out the statistical analyses. JJ, SA, and TCT drafted the manuscript, with major input from WK.

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Correspondence to Wonyong Kim.

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Our research did not include any human subjects and animal experiments.

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Jeong, J., Ahn, S., Truong, T.C. et al. Description of Mycolicibacterium arenosum sp. nov. Isolated from Coastal Sand on the Yellow Sea Coast. Curr Microbiol 81, 73 (2024). https://doi.org/10.1007/s00284-023-03587-4

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  • DOI: https://doi.org/10.1007/s00284-023-03587-4

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