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Mutanase from Trichoderma harzianum inductively Produced by Mutan: Short-Term Treatment to Degrade Mature Streptococcus mutans Biofilm

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Abstract

This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutans biofilms (n = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilms were analyzed by amount of matrix water-insoluble polysaccharides, bacterial viability, acidogenicity, and morphology by scanning electron microscopy (SEM). The group treated with active enzymes (AE) had a significantly lower amount of insoluble polysaccharides (893.30 ± 293.69) when compared to the negative control group (NaCl, 2192.59 ± 361.96), yet no significant difference was found when comparing to the positive control group (CHX, 436.82 ± 151.07). Also, there was no significant effect on bacteria metabolism and viability (P-value < 0.05). Data generated by the quantitative analysis were confirmed through scanning electron microscopy images. Thus, fungal mutanase degraded the biofilm after a short-term treatment without interfering with bacterial viability and metabolism. Such findings offer insight to the development of routine oral care products containing this input.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Prof. Dr. Jaime Aparecido Cury and Prof. Dr. Livia Maria Andaló Tenuta from the Piracicaba Dental School-UNICAMP (São Paulo, Brazil) for providing Streptotoccus mutans UA159. We also thank Dr Rodrigo Ferreira Silva from the Laboratory of Scanning Electronic Microscopy and Elementary Microanalysis (Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Brazil) for technical assistance during the acquisition of SEM images and Maria Gerusa Brito Aragão for the critical review of the manuscript. This work was funded by the São Paulo Research Foundation (FAPESP) [Grant Numbers 2019/19162-7 and 2020/07315-0].

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São Paulo Research Foundation (FAPESP) [Grant Numbers 2019/19162–7 and 2020/07315–0].

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Authors and Affiliations

  1. Department of Children’s Clinic, Ribeirão Preto School of Dentistry, University of São Paulo, Café Avenue s/n, Ribeirão Preto, São Paulo, 14040-904, Brazil

    Jéssica Silva Peixoto Bem

  2. Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Café Avenue s/n, Ribeirão Preto, São Paulo, 14040-903, Brazil

    Nayanna Gomes Silva Lacerda, Ana Cristina Morseli Polizello & Carolina Patrícia Aires

  3. Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Café Avenue s/n, Ribeirão Preto, São Paulo, 14040-903, Brazil

    Hamilton Cabral & Nathalia Gonsales da Rosa-Garzon

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  1. Jéssica Silva Peixoto Bem
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  3. Ana Cristina Morseli Polizello
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JSPB, HC, CPA: conceptualization and methodology; JSPB, NGSL, ACMP, NGdR-G: investigation and formal analysis; JSPB, NGSL, ACMP: writing—original draft; NGdR-G, HC, CPA: writing—review & editing. All authors approved the final submitted version.

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Correspondence to Carolina Patrícia Aires.

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Bem, J.S.P., Lacerda, N.G.S., Polizello, A.C.M. et al. Mutanase from Trichoderma harzianum inductively Produced by Mutan: Short-Term Treatment to Degrade Mature Streptococcus mutans Biofilm. Curr Microbiol 80, 312 (2023). https://doi.org/10.1007/s00284-023-03417-7

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