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Alkalihalobacillus deserti sp. nov., Isolated from the Saline–Alkaline Soil

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Abstract

A bacterial strain, designated TRPH29T, was isolated from saline-alkaline soil, collected from the southern edge of the Gurbantunggut desert, Xinjiang, People’s Republic of China. The isolate was Gram-staining positive, facultatively anaerobic, straight rods. Growth occurred at 15–40 °C (optimum, 28 °C), pH 8.0–13.0 (optimum, 10.0), and in the presence of 0–15% (w/v) NaCl (optimum, 2%). Phylogenetic analysis using 16S rRNA gene sequence indicated that strain TRPH29T showed the highest sequence similarities to Alkalihalobacillus krulwichiae (98.31%), Alkalihalobacillus wakoensis (98.04%), and Alkalihalobacillus akibai (97.69%). Average nucleotide identity (ANI) and digital DNA-DNA hybridization values between strain TRPH29T and Alkalihalobacillus krulwichiae, Alkalihalobacillus wakoensis, Alkalihalobacillus akibai were in the range of 73.62–75.52% and 15.0–21.20%, respectively. Results of genome analyses indicated that the genome size of strain TRPH29T was 5.05 Mb, with a genomic DNA G + C content of 37.30%. Analysis of the cellular component of strain TRPH29T revealed that the primary fatty acids were anteiso-C15:0 and iso-C15:0, and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid, and an unidentified phospholipid. The predominant respiratory quinone was MK-7. Based on the genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain TRPH29T represents a novel species of the genus Alkalihalobacillus, for which the name Alkalihalobacillus deserti sp. nov. is proposed. The type strain is TRPH29T (= CGMCC 1.19067T = NBRC 115475T).

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Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

ML:

Maximum-likelihood

NJ:

Neighbor-joining

GTDB-Tk:

Genome taxonomy database‐toolkit

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Acknowledgements

The authors are grateful to Professor Yan Zhang (School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China) for kindly providing the reference type strains. We also thank Prof. Aharon Oren from Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem for his kindly help with nomenclature of the new taxa.

Funding

This work was supported by National Natural Science Foundation of China (Nos. 31570109 and 32061143043), Scientific projects of colleges and universities in Xinjiang Autonomous Region (XJEDU2018I016) and the open subject of the Key Laboratory of the Autonomous Region (02017D0408). Wen-Jun Li was also supported by the Third Xinjiang Scientific Expedition Program (Grant No. 2022xjkk1204).

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Authors and Affiliations

  1. Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, People’s Republic of China

    Zulihumaer Rouzi, Muyesaier Aosiman, Dilireba Abudourousuli & Deng-Di An

  2. CAS Key Laboratory of Tropical Marine Bio Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 511458, People’s Republic of China

    Yu-Qian Li

  3. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Lan Liu & Wen-Jun Li

  4. Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh, 11564, Kingdom of Saudi Arabia

    Dalal Hussien M. Alkhalifah

  5. Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein

  6. Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt

    Wael N. Hozzein

  7. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Wen-Jun Li

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  1. Zulihumaer Rouzi
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Contributions

DDA and WJL designed the work. ZR, MA and DA carry out the morphological, biochemical, physiological and molecular characterisation of novel strain and maintained the bacterial cultures. YQL and LL carried out the genomic data retrieval from databases, phylogenomic, phylogenetic data analysis. ZR, YQL, DHMA and WNHJ carried out the article examination. All authors contributed to writing the manuscript and accepted it for publication.

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Correspondence to Deng-Di An or Wen-Jun Li.

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Rouzi, Z., Li, YQ., Aosiman, M. et al. Alkalihalobacillus deserti sp. nov., Isolated from the Saline–Alkaline Soil. Curr Microbiol 80, 261 (2023). https://doi.org/10.1007/s00284-023-03353-6

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