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Influence of Cell Wall on Biomolecules Biosynthesis in Chlamydomonas reinhardtii Strains Exposed to Magnetic Fields

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Abstract

The application of magnetic fields (MF) has attracted the attention of researchers due to their efficiency to change cell metabolism. Chlamydomonas reinhardtii is a biotechnologically useful microalga with versatile metabolism that may be a valuable organism to study the effects of the MF in biology. Therefore, two C. reinhardtii strains, one with cell wall (2137) and other which lacks the cell wall (Wt-S1—cc4694), were evaluated that a new sensitivity factor in the analysis could be included. Comparative studies were undertaken with the two C. reinhardtii strains under the MF intensities of 0.005 mT (terrestrial MF - control), 11  and 20 mT. Results indicated that the physical cell wall barrier protected cells against the MF applied during the assays. Only with the highest MF applied (20 mT) a slight increase in lipid concentration in the cell wall strain was detected. The lowest growth of the strain that lacks cell wall (Wt-S1) indicated that these cells are under a negative effect. To cope with the two MF stresses conditions, Wt-S1 cells produced more pigments (chlorophylls and carotenoids) and lipids and enhanced the antioxidant defense system. The raise of these compounds under MF could potentially have a positive biotechnological impact on algal biomass.

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Data Availability

The data presented in this study are available upon request from the corresponding author.

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Acknowledgements

The authors are thankful to the National Council for Scientific and Technological Development (CNPq-Brazil) and Fundação para a Ciência e Tecnologia (FCT-Portugal). This study was partially funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil, Finance Code 001.

Funding

The present study was financed by CNPq - Conselho Nacional de Desenvolvimento Científco e Tecnológico supported) and Fundação para a Ciência e Tecnologia (FCT-Portugal), through the research units LEAF (UID/AGR/04129/2020) and the FCT/CAPES transnational cooperation FCT-2367/CAPES-10343/13.

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  1. Maria da Gloria Esquível

    Present address: Landscape, Environment, Agriculture and Food – LEAF Centre, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017, Lisboa, Portugal

Authors and Affiliations

  1. Laboratory of Biotechnology, Chemistry and Food School, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil

    Lenon M. Bauer & Lucielen O. Santos

  2. Laboratory of Biochemical Engineering, Chemistry and Food School, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil

    Jorge Alberto V. Costa & Ana Priscila C. da Rosa

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  1. Lenon M. Bauer
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  2. Maria da Gloria Esquível
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  5. Lucielen O. Santos
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Contributions

LMB; MGE; JAVC; APC R and LOS contributed to the study conception and design. Material preparation, data collection and analysis were performed by LMB. The first draft of the manuscript was written by LMB and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Lucielen O. Santos.

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Bauer, L.M., da Gloria Esquível, M., Costa, J.A.V. et al. Influence of Cell Wall on Biomolecules Biosynthesis in Chlamydomonas reinhardtii Strains Exposed to Magnetic Fields. Curr Microbiol 80, 96 (2023). https://doi.org/10.1007/s00284-023-03189-0

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