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Neoroseomonas marina sp. nov., Isolated from a Beach Sand

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Abstract

A pink-pigmented bacterium (strain JC162T = KCTC 32190T) was isolated from a beach sand sample. Cells were Gram-stain-negative, coccoid, non-motile, and strictly aerobic. EzBioCloud BLAST search of 16S rRNA gene sequence showed that strain KCTC 32190T had the highest sequence identity to the members of the genus Neoroseomonas and was closely related to N. oryzicola YC6724T (99.8%), N. sediminicola FW-3T (98.5%), N. soli 5N26T (98.2%), and other members of the genus Neoroseomonas (< 97.9%) in the family Acetobacteriaceae within the class of Alphaproteobacteria. Chemo-organoheterotrophy was the only growth mode and growth was possible on a wide range of organic substrates. Strain KCTC 32190T was positive for catalase and oxidase. Fatty acid composition of strain KCTC 32190T includes (in decreasing %) C18:1ω7c, cyclo-C19:0ω8c, C18:02-OH, C16:0, C18:03-OH, C16:1ω7c/C16:1ω6c, C16:02-OH and C16:1ω5c. Polar lipids comprised of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified amino lipid, and three unidentified lipids. The genomic DNA G+C content of the strain KCTC 32190T was 70.9 mol%. Strain KCTC 32190T has a low ANI value of < 92.7% and genome reassociation (based on digital DNA-DNA hybridization) value of < 48.8% with the nearest type strains. The genome relatedness is supported by other polyphasic taxonomic data to propose strain KCTC 32190T as a new species in the genus Neoroseomonas with the name Neoroseomonas marina sp. nov. The type strain is strain JC162T (KCTC 32190T = CGMCC1.12364T).

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Abbreviations

NCBI:

National Center for Biotechnology Information

ANI:

Average Nucleotide Identity

dDDH:

Digital DNA-DNA Hybridization

BLAST:

Basic Local Alignment Search Tool

MUSCLE:

Multiple Sequence Comparison by Log-Expectation

KCTC:

Korean Collection for Type Cultures

CGMCC:

China General Microbiological Culture Collection Centre

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Acknowledgements

Anusha thanks DST, Government of India for the award of INSPIRE Senior Research Fellowship. Ramana thanks the Department of Biotechnology, Government of India for the award of Tata Innovation Fellow. Financial support received from CSIR, IoE UoH and BUILDER UoH is acknowledged. Sasikala thanks UGC for the award of mid-career grant. The culture was initially isolated by Mr. B. Parag for which he is acknowledged. Infrastructural facilities funded by DST-FIST (UoH and JNTUH), UGC-SAP (DRS; UoH), TEQIP and AICTE (from JNTUH) are acknowledged.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India

    Anusha Rai, Deepshikha Gupta, Smita Nandardhane, Ipsita Sahu & Venkata Ramana Chintalapati

  2. Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad, 500 085, India

    Jagadeeshwari Uppada & Sasikala Chintalapati

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Contributions

AR, JU, DS designed the study under the supervision of SC and RCV. AR, SN, and IS performed isolation, deposition, and identification. AR, JU, and DS performed genomic analysis. AR and DS drafted the manuscript, SC and RCV reviewed and finalised MS. All authors read and approved the final manuscript.

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Correspondence to Sasikala Chintalapati or Venkata Ramana Chintalapati.

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Rai, A., Uppada, J., Gupta, D. et al. Neoroseomonas marina sp. nov., Isolated from a Beach Sand. Curr Microbiol 79, 233 (2022). https://doi.org/10.1007/s00284-022-02917-2

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