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Ammonium Removal by a Newly Isolated Heterotrophic Nitrification–Aerobic Denitrification Bacteria Pseudomonas Stutzeri SDU10 and Its Potential in Treatment of Piggery Wastewater

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Abstract

A strain SDU10 was isolated from swine manure compost and identified as Pseudomonas stutzeri SDU10. It demonstrated excellent capability in NH4+-N removal. Optimal conditions of NH4+-N removal were determined, which were sodium acetate as the optimal carbon source, carbon to nitrogen (C/N) ratio of 10, temperature of 30 °C, pH of 7.0. Especially, P. stutzeri SDU10 could remove high concentration NH4+-N of 1500.0 and 2000.0 mg/l in 120 h with the NH4+-N removal rates of 91.1% and 61.6%, respectively. In batch experiments, the highest NH4+-N removal rate of 97.6% and chemical oxygen demand (COD) removal rate of 94.2% were obtained at initial C/N ratio 10 during piggery wastewater treatment using P. stutzeri SDU10. Results showed that P. stutzeri SDU10 had the potential for treatment of wastewater of high NH4+-N concentration.

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Acknowledgements

This research was supported by the Project of Taishan Industry Leading Talent of Shandong province, China (LJNY201603). The authors would like to thank Chengjia Zhang, Caiyun Sun from the Core Facilities for Life and Environmental Sciences, State Key Lab of Microbial Technology for help and guidance in sample analyses.

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, 266237, PR China

    Lifei Chen, Jianqiang Lin, Linxu Chen & Jianqun Lin

  2. Shandong Engineering Laboratory of Treatment and Resource Utilization of Waste From Planting and Breeding Industry, Shandong Yian Bioengineering Co., Ltd, Jinan, 250014, PR China

    Deng Pan

  3. Qingdao Longding Biotech Co., Ltd, Qingdao, 266109, PR China

    Yilin Ren

  4. Shandong Institute for Product Quality Inspection, Jinan, 250102, PR China

    Juan Zhang

  5. College of Life Sciences, Shandong Agricultural University, Taian, 271018, PR China

    Bo Zhou

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  1. Lifei Chen
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Contributions

LF C, JQ L, and JQ L designed the experiments. LF C and LX C performed the experiments. D P, YL R, J Z, and B Z gave support on the experiments. LF C, LX C, JQ L, and JQ L analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Linxu Chen or Jianqun Lin.

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This article does not contain any studies with human participants or animals performed by any of the authors. The wastewater collected in this experiment has been approved by the owner of Miaoshi pig farm and Qingdao Municipal Animal Husbandry Bureau.

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Chen, L., Lin, J., Pan, D. et al. Ammonium Removal by a Newly Isolated Heterotrophic Nitrification–Aerobic Denitrification Bacteria Pseudomonas Stutzeri SDU10 and Its Potential in Treatment of Piggery Wastewater. Curr Microbiol 77, 2792–2801 (2020). https://doi.org/10.1007/s00284-020-02085-1

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