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Characterization of New Fengycin Cyclic Lipopeptide Variants Produced by Bacillus amyloliquefaciens (ET) Originating from a Salt Lake of Eastern Algeria

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Abstract

Fengycin antibiotic displays a strong antifungal activity and inhibits the growth of a wide range of plant pathogens especially filamentous fungi. The main objective of the present study is to characterize fengycin variants produced by B. amyloliquefaciens strain (ET). LC–MS analysis of fengycin extracts has shown several molecular ion peaks corresponding to conventional fengycin homologues (MH + : m/z 1463.9; 1491.9; 1506) and some new ones (MH + : m/z 1433; 1447; 1461; and 1477). Further characterization of these precursor ions was carried out by LC–MS.MS analysis. Reporter fragment ions were observed (named A and B), they correspond to the cleavage of Orn2-Tyr3 (A), Glu1-Orn2 (B), and used for identifying fengycin variants. The reporter fragment couple ions [A/B] at [m/z 966.5/1080.5] and [m/z 994.4 /1108.5] represent fengycin A and B, respectively. The diagnostic ions at ([m/z 980/1094]) may correspond to fengycin C3, D, S or B2. Interestingly, unknown diagnostic product ions at [m/z 951/1065] and [m/z 979/1093] were detected for the first time in this study which prove that they correspond to new fengycin variants, named fengycin X and fengycin Y, respectively. The fengycin X results from a substitution of the glutamine amino acid (Q), at position 8 of the fengycin A peptide part, by an isoleucine (I) or a leucine (L) residue. This mutation should be the same in fengycin Y but compared to fengycin B.

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Acknowledgments

The authors are grateful to a fellowships European program Erasmus Mundus External Cooperation Window-consortium AVERROES

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Authors and Affiliations

  1. Faculty of Sciences, Department of Biology, University of M’Hamed Bougara, 35000, Boumerdès, Algeria

    Asma Ait Kaki

  2. Bioindustries Unit, Gembloux Agrobiotech, University of Liege, Passage of the Deportees 2, 5030, Gembloux, Belgium

    Asma Ait Kaki & Marc Ongena

  3. Mass Spectrometry Laboratory, Molecular Systems Research Unit, University of Liege, B6C, B 400, Liege, Belgium

    Nicolas Smargiasso & Edwin De Pauw

  4. Department of Animal Productions, Faculty of Veterinary Medicine, University of Liege, Colonster Boulevard 20, 4000, Liege, Belgium

    Nassim Moula

  5. Laboratory of Mycology, Biology and Biotechnology, Faculty of Natural and Life Sciences, Department of Applied Biology, University of Mentouri 1, 25000, Constantine, Algeria

    Asma Ait Kaki, Mounira Kara Ali & Noreddine Kacem Chaouche

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  1. Asma Ait Kaki
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  2. Nicolas Smargiasso
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Ait Kaki, A., Smargiasso, N., Ongena, M. et al. Characterization of New Fengycin Cyclic Lipopeptide Variants Produced by Bacillus amyloliquefaciens (ET) Originating from a Salt Lake of Eastern Algeria. Curr Microbiol 77, 443–451 (2020). https://doi.org/10.1007/s00284-019-01855-w

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