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Complete Genome Sequence of Paenibacillus sp. CAA11: A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing

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Abstract

Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from lignocellulosic biomass. Successful CBP, an efficient and economical lignocellulosic biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp. CAA11, a newly isolated promising microbial host for CBP-producing ethanol and organic acids from cellulose. The genome of Paenibacillus sp. CAA11 comprises one 4,888,410 bp chromosome with a G + C content of 48.68% containing 4418 protein-coding genes, 102 tRNA genes, and 39 rRNA genes. The functionally active cellulase, encoded by CAA_GH5 was identified to belong to glycosyl hydrolase family 5 (GH5) and consisted of a catalytic domain and a cellulose-binding domain 3 (CBM3). When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a previously verified powerful bacterial cellulase. This study demonstrates the potential of Paenibacillus sp. CAA11 as a CBP-enabling microbe for cost-effective biofuels/chemicals production from lignocellulosic biomass.

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Acknowledgements

This research was supported by C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT (Information & Communication Technology) & Future Planning (2016M3D3A1A01913249). The authors also appreciate further support by Korea Institute of Science and Technology (KIST) Institutional Program (2E28290).

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Author notes

  1. Gyeongtaek Gong and Hyun Ju Oh are co-first authors.

Authors and Affiliations

  1. Clean Energy Research Center, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792, Republic of Korea

    Gyeongtaek Gong, Hyun Ju Oh, Sukhyeong Cho, Youngsoon Um & Sun-Mi Lee

  2. Department of Chemical and Biological Engineering, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, Republic of Korea

    Hyun Ju Oh & Min-Kyu Oh

  3. Division of Chemical and Medical Metrology, Center for Bioanalysis, Korea Research Institute of Standards and Science, 267 Gajeong-Ro, Yuseong-Gu, Daejeon, 34113, Republic of Korea

    Seil Kim

  4. Division of Energy and Environmental Technology, University of Science and Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea

    Youngsoon Um & Sun-Mi Lee

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  1. Gyeongtaek Gong
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Correspondence to Sun-Mi Lee.

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Gong, G., Oh, H.J., Cho, S. et al. Complete Genome Sequence of Paenibacillus sp. CAA11: A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing. Curr Microbiol 76, 732–737 (2019). https://doi.org/10.1007/s00284-019-01685-w

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