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Clustering of Clinical and Environmental Escherichia coli O104 Isolates Using the DiversiLab™ Repetitive Sequence-Based PCR System

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Abstract

The DiversiLab™ rep-PCR system was used to amplify DNA regions of 28 well-characterized Escherichia coli O104 strains to generate a digital DNA fingerprint profile for strain differentiation. E. coli O104 strains from human stools and other sources were examined. The results indicate that this system can cluster similar O104 strains rapidly.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biological Sciences, California State University, East Bay, 25800 Carlos Bee Blvd., Hayward, CA, 94542, USA

    N. M. Herbold & C. R. Lauzon

  2. MagArray, Inc., 1230 Bordeaux Dr., Sunnyvale, CA, 94089, USA

    L. M. Clotilde

  3. U.S. Food and Drug Administration, Office of Regulatory Affairs, San Francisco District Laboratory, 1431 Harbor Bay Pkwy., Alameda, CA, 94502, USA

    K. M. Anderson, G. L. Hartman & A. Lin

  4. U. S. Food and Drug Administration, Center for Food Safety and Applied Nutrition Office of Regulatory Science, 5100 Paint Branch Pkwy., College Park, MD, 20740, USA

    J. Kase

  5. Division of Field Sciences, U. S. Food and Drug Administration, Office of Regulatory Affairs, 2094 Gaither Rd., Rockville, MD, 20850, USA

    S. Himathongkham

Authors
  1. N. M. Herbold
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  2. L. M. Clotilde
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  3. K. M. Anderson
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  4. J. Kase
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  5. G. L. Hartman
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  6. S. Himathongkham
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  7. A. Lin
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  8. C. R. Lauzon
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Correspondence to C. R. Lauzon.

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This manuscript does not contain clinical studies or patient data.

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Herbold, N.M., Clotilde, L.M., Anderson, K.M. et al. Clustering of Clinical and Environmental Escherichia coli O104 Isolates Using the DiversiLab™ Repetitive Sequence-Based PCR System. Curr Microbiol 70, 436–440 (2015). https://doi.org/10.1007/s00284-014-0728-z

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  • DOI: https://doi.org/10.1007/s00284-014-0728-z

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